{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T21:47:12Z","timestamp":1780696032979,"version":"3.54.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T00:00:00Z","timestamp":1588636800000},"content-version":"vor","delay-in-days":125,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004763","name":"Natural Science Foundation of Inner Mongolia Autonomous Region","doi-asserted-by":"publisher","award":["2016MS08106"],"award-info":[{"award-number":["2016MS08106"]}],"id":[{"id":"10.13039\/501100004763","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81660048"],"award-info":[{"award-number":["81660048"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81760056"],"award-info":[{"award-number":["81760056"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Background and Objectives<\/jats:italic>. <jats:italic>Dracocephalum moldavica L.<\/jats:italic> is an herbal medicine used to treat cardiovascular diseases in China. The purpose of this study was to assess the cardioprotective effect of <jats:italic>D. moldavica L<\/jats:italic>. extracts against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced apoptosis and oxidative stress in H9c2 cells and to explore the mechanism behind this effect. <jats:italic>Materials and Methods<\/jats:italic>. The petroleum ether (petrol), dichloromethane (CH<jats:sub>2<\/jats:sub>Cl<jats:sub>2<\/jats:sub>), ethyl acetate (EtOAc), and n\u2010butyl alcohol (n\u2010BuOH) fractions were isolated from alcohol extracts of <jats:italic>D<\/jats:italic>. <jats:italic>moldavica L<\/jats:italic>. Total phenolic and flavonoid contents and <jats:italic>in vitro<\/jats:italic> antioxidant activities of different fractions were evaluated. H9c2 cells were then treated with <jats:italic>D<\/jats:italic>. <jats:italic>moldavica L<\/jats:italic>. extracts before challenging with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. Cell viability was determined by colorimetric assay, and ELISA was used to measure the levels of lactate dehydrogenase (LDH), malondialdehyde (MDA), and superoxide dismutase (SOD). Apoptosis levels and mitochondrial membrane potential were measured by flow cytometry. The expressions of cell apoptosis regulatory proteins caspase\u20103, Bax, and Bcl\u20102 were determined by western blotting. <jats:italic>Results<\/jats:italic>. Our results demonstrated that the EtOAc fraction from <jats:italic>D. moldavica L<\/jats:italic>. ethanol extract, which is rich in phenolic and flavonoid active constituents, had the strongest free radical scavenging activity. Additionally, this fraction increased H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced reduction in cell viability, SOD activity, and mitochondrial membrane potential. It also reduced H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced elevation in ROS production, contents of LDH and MDA, and H9c2 apoptosis. We further found that the EtOAc fraction increased Bcl\u20102 expression, while it decreased caspase\u20103 and Bax expressions induced by H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> in H9c2 cells. <jats:italic>Conclusions<\/jats:italic>. Our data revealed that the EtOAc fraction from <jats:italic>D. moldavica L<\/jats:italic>. ethanol extract ameliorates H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010induced cardiotoxicity via antiapoptotic and antioxidant mechanisms.<\/jats:p>","DOI":"10.1155\/2020\/8379358","type":"journal-article","created":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T23:52:33Z","timestamp":1588722753000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["<i>Dracocephalum moldavica L<\/i>. 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