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This study aimed to provide baseline information for future artemisinin surveillance by analyzing the <jats:italic>k13<\/jats:italic>\u2010propeller domain in <jats:italic>P. falciparum<\/jats:italic> field isolates collected from 24 study areas in 14 malaria hot spots of Odisha (previously Orissa) during July 2018\u2010January 2019. A total of 178 <jats:italic>P. falciparum<\/jats:italic> mono infections were assessed. An 849\u2010base pair fragment encoding the <jats:italic>Pfk13<\/jats:italic> propeller was amplified by nested polymerase chain reaction and sequenced in both directions (PCR). After DNA alignment with the 3D7 reference sequence, all samples were found to be wild type. It can be anticipated that malaria public health is not under direct threat in Odisha relating to ART resistance.<\/jats:p>","DOI":"10.1155\/2020\/8475246","type":"journal-article","created":{"date-parts":[[2020,3,16]],"date-time":"2020-03-16T23:32:11Z","timestamp":1584401531000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Sequence Analysis of the <i>K13<\/i>\u2010Propeller Gene in Artemisinin Challenging <i>Plasmodium falciparum<\/i> Isolates from Malaria Endemic Areas of Odisha, India: A Molecular Surveillance Study"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6406-9371","authenticated-orcid":false,"given":"Ramakanta","family":"Rana","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8415-6701","authenticated-orcid":false,"given":"Manoranjan","family":"Ranjit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhusmita","family":"Bal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemant Kumar","family":"Khuntia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7717-5592","authenticated-orcid":false,"given":"Sanghamitra","family":"Pati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sri","family":"Krishna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4081-5816","authenticated-orcid":false,"given":"Aparup","family":"Das","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,3,16]]},"reference":[{"volume-title":"Guidelines for the Treatment of Malaria. 3rd edition","year":"2015","author":"World Health Organization","key":"e_1_2_8_1_2"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe1604520"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0808859"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-019-2701-6"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.161"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2624"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01578-16"},{"volume-title":"Global Malaria Programme, status report on artemisinin resistance, and artemisinin based combination therapy efficacy","year":"2018","author":"World Health Organization","key":"e_1_2_8_8_2"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy1038"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40794-016-0027-8"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.16-0234"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpddr.2012.01.001"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI21682"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14127"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(12)60484-x"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuw037"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2305062"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70252-4"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2624"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12876"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4381"},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00354-18"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0213686"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1713777"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-016-1636-4"},{"key":"e_1_2_8_26_2","first-page":"289","article-title":"Malaria in Odisha and future perspectives","volume":"114","author":"Dhangadamajhi G.","year":"2015","journal-title":"The Journal of Infectious Diseases"},{"volume-title":"Molecular Cloning\u2013A Laboratory Manual","year":"2001","author":"Brook J. 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