{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:11:02Z","timestamp":1785363062863,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T00:00:00Z","timestamp":1594857600000},"content-version":"vor","delay-in-days":197,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81430099"],"award-info":[{"award-number":["81430099"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["91231206"],"award-info":[{"award-number":["91231206"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Objects<\/jats:italic>. To investigate the lncRNA\u2010mediated <jats:italic>trans<\/jats:italic>\u2010 and <jats:italic>cis<\/jats:italic>\u2010regulation of genes expression underlying leukocyte functions and characteristics, especially the leukocyte\u2010related biomarkers implicated in the linkage between traditional Chinese medicine\u2010 (TCM\u2010) defined qi\u2010deficiency constitution (QDC) and Pi\u2010qi\u2010deficiency syndrome (PQDS) of chronic superficial gastritis (CSG). <jats:italic>Methods<\/jats:italic>. We adopted RNA\u2010sequencing approach to identify differential lncRNAs and genes in leukocytes, clustered expression profiles, and analyzed biological functions and pathways of differential genes to decode their potential roles in contributing to characteristics and functions of leukocytes. In addition, interaction networks were created to detail the interactions between differential genes. In particular, we explored differential lncRNAs\u2010mediated regulation of differential genes and predicted the subcellular location of lncRNAs to reveal their potential roles. <jats:italic>Results<\/jats:italic>. Compared with TCM\u2010defined balanced constitution (BC), 183 and 93 genes as well as 749 and 651 lncRNAs were differentially expressed (<jats:italic>P<\/jats:italic> &lt; 0.05 and |log<jats:sub>2<\/jats:sub> (fold change)| \u22651) in leukocytes of individuals from case populations 1 (QDC) and 2 (PQDS), respectively. Of them, 12 genes and 111 lncRNAs were common to each case population. Several networks were created to detail the interactions among case\u2010specific genes, especially case\u2010specific lncRNAs\u2010mediated regulation of case\u2010specific genes. Also, interaction networks were created for the common lncRNAs and genes. HCL analyses showed that differential genes and lncRNAs, especially the common genes and lncRNAs, kept similar expression patterns in both case populations. Furthermore, function enrichment analyses just indicated the common biological processes, namely, extracellular matrix organization and cell adhesion via plasma membrane adhesion molecules. In addition, most common genes underwent very tight and complex regulation of many <jats:italic>trans<\/jats:italic>\u2010 and <jats:italic>cis<\/jats:italic>\u2010acting lncRNAs. In particular, of them, ADAMTSL5, COL26A1, COL27A1, MSH5, and LOC390937 could be regulated by multiple case\u2010specific and common lncRNAs, including the means that directs binding of the common lncRNAs to their coded proteins. The common changes in the extracellular matrix and integral components of plasma membrane related to cell\u2010cell adhesion\/junction and communication may implicate the linkage between QDC and PQDS, contributing to alterations in characteristics and functions of leukocytes. <jats:italic>Conclusions<\/jats:italic>. These results may provide new insights into the characteristic and functional changes of leukocytes in QDC and PQDS, especially the mechanism underlying the linkage of QDC to PQDS, with potential leukocytes biomarkers for future application in integrative medicine.<\/jats:p>","DOI":"10.1155\/2020\/8518053","type":"journal-article","created":{"date-parts":[[2020,7,16]],"date-time":"2020-07-16T23:33:19Z","timestamp":1594942399000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Integrated Analyses of lncRNA and mRNA Profiles Reveal Characteristic and Functional Changes of Leukocytes in Qi\u2010Deficiency Constitution and Pi\u2010Qi\u2010Deficiency Syndrome of Chronic Superficial Gastritis"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8120-8916","authenticated-orcid":false,"given":"Leiming","family":"You","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7628-5669","authenticated-orcid":false,"given":"Aijie","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaopu","family":"Sang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhui","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting\u2019An","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunyu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangrui","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2948-3507","authenticated-orcid":false,"given":"Anlong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,16]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/480s94a"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/9439682"},{"key":"e_1_2_12_3_2","first-page":"1","article-title":"Classification and diagnosis basis of nine basic constitutions in Chinese medicine","volume":"28","author":"Wang Q.","year":"2005","journal-title":"Journal of Traditional Chinese Medical Sciences"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11684-012-0173-y"},{"key":"e_1_2_12_5_2","volume-title":"Traditional Chinese Medicine Constitutionology in 2008","author":"Wang Q.","year":"2009"},{"key":"e_1_2_12_6_2","first-page":"311","article-title":"Primary exploration on immune associated genome of patients with pi-qi deficiency syndrome","volume":"25","author":"Luo Y. J.","year":"2005","journal-title":"Chinese Journal of Integrated Traditional and Western Medicine"},{"key":"e_1_2_12_7_2","first-page":"1427","article-title":"Metabonomics study on urine 1H-NMR in chronic superficial gastritis patients with pi-qi deficiency syndrome\/pi-wei dampness-heat syndrome","volume":"35","author":"Shi X. G.","year":"2015","journal-title":"Chinese Journal of Integrated Traditional and Western Medicine"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-009-0341-3"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201700027"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-017-1215-1"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0188-9"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02229-1"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.18632\/aging.102164"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11081170"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.06.009"},{"key":"e_1_2_12_16_2","volume-title":"Classification and Determination of Constitution in Tradtional Chinese Medicine","author":"China Association of Chinese Medicine","year":"2009"},{"key":"e_1_2_12_17_2","volume-title":"Guiding Principle for Clinical Research on New Drugs of Traditional Chinese Medicine","author":"Zheng X. Y.","year":"2002"},{"key":"e_1_2_12_18_2","first-page":"44","article-title":"China chronic gastritis Consensus (2012, Shanghai)","volume":"5","author":"Fang J. Y.","year":"2013","journal-title":"Chinese Journal of Integrative Medicine"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bth078"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr483"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.1039\/c5mb00663e"},{"key":"e_1_2_12_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_2_12_27_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_12_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1107"},{"key":"e_1_2_12_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.09.001"},{"key":"e_1_2_12_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000617"},{"key":"e_1_2_12_31_2","article-title":"RIsearch2: suffix array-based large-scale prediction of RNA-RNA interactions and siRNA off-targets","volume":"45","author":"Alkan F.","year":"2017","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_12_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty085"},{"key":"e_1_2_12_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-489"},{"key":"e_1_2_12_34_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq1069"},{"key":"e_1_2_12_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcms.2017.08.005"},{"key":"e_1_2_12_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcms.2014.09.005"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/8518053.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/8518053.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/8518053","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T07:43:54Z","timestamp":1723016634000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/8518053"}},"subtitle":[],"editor":[{"given":"Mark","family":"Moss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/8518053"],"URL":"https:\/\/doi.org\/10.1155\/2020\/8518053","archive":["Portico"],"relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"value":"1741-427X","type":"print"},{"value":"1741-4288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2019-12-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-06-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8518053"}}