{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:48:41Z","timestamp":1787017721890,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T00:00:00Z","timestamp":1598313600000},"content-version":"vor","delay-in-days":237,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004919","name":"King Abdulaziz City for Science and Technology","doi-asserted-by":"publisher","award":["14-MED333-10"],"award-info":[{"award-number":["14-MED333-10"]}],"id":[{"id":"10.13039\/501100004919","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    This study deals with\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010glucosidase and\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010secretase inhibitory screening of extract\/fractions and isolated daturaolone (\n                    <jats:bold>1<\/jats:bold>\n                    ), namely, 3\u2010oxo\u20106\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010hydroxy\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010amyrin (daturaolone) from chloroform fraction of\n                    <jats:italic>Datura metel<\/jats:italic>\n                    L. Among entire fractions, the chloroform soluble fraction showed excellent activity against\n                    <jats:italic>\u03b1<\/jats:italic>\n                    \u2010glucosidase with % inhibition 90.8 with IC\n                    <jats:sub>50<\/jats:sub>\n                    160.2 \u00b1 1.85\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g and daturaolone (\n                    <jats:bold>1<\/jats:bold>\n                    ) with 98.7% inhibition with IC\n                    <jats:sub>50<\/jats:sub>\n                    840.4 \u00b1 1.74\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M, respectively. Similarly, extract and daturaolone (\n                    <jats:bold>1<\/jats:bold>\n                    ) also exhibited significant activity against the\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010secretase enzyme (BACE1) with % activities 88.27 and 95.19 and with IC\n                    <jats:sub>50<\/jats:sub>\n                    values 304.21 \u00b1 2.98\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g and 260.70 \u00b1 1.87\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M, respectively, as compared to the standard inhibitor (Ans670, Sta671, Val672)\u2010amyloid\u2010\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \/A4 precursor protein 770 fragments 662\u2010675) with % activity 94.21 and IC\n                    <jats:sub>50<\/jats:sub>\n                    value 289.24 \u00b1 1.60\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M. This finding encourages and opens a new window for further detail phytochemical investigation on\n                    <jats:italic>D<\/jats:italic>\n                    .\n                    <jats:italic>metel<\/jats:italic>\n                    in order to isolate novel compounds with promising enzyme inhibitory potential.\n                  <\/jats:p>","DOI":"10.1155\/2020\/8530165","type":"journal-article","created":{"date-parts":[[2020,8,25]],"date-time":"2020-08-25T19:32:33Z","timestamp":1598383953000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Potent\n                    <i>In Vitro \u03b1<\/i>\n                    \u2010Glucosidase and\n                    <i>\u03b2<\/i>\n                    \u2010Secretase Inhibition of Amyrin\u2010Type Triterpenoid Isolated from\n                    <i>Datura metel<\/i>\n                    Linnaeus (Angel\u2019s Trumpet) Fruits"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2800-1545","authenticated-orcid":false,"given":"Saud","family":"Bawazeer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2429-5491","authenticated-orcid":false,"given":"Abdur","family":"Rauf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3140-5153","authenticated-orcid":false,"given":"Sami","family":"Bawazeer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,25]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/fedr.19870980707"},{"key":"e_1_2_10_2_2","first-page":"568","article-title":"A novel antifungal pyrrole derivative from Datura metel leaves","volume":"59","author":"Dabur R.","year":"2004","journal-title":"Pharmazie"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3657-09.2009"},{"key":"e_1_2_10_4_2","first-page":"277","article-title":"Isolation, characterization and antibacterial activity of alkaloid from Datura metel Linn leaves","volume":"3","author":"Okwu D. 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