{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:05:01Z","timestamp":1790633101793,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T00:00:00Z","timestamp":1587427200000},"content-version":"vor","delay-in-days":111,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>The emergence and resurgence of <jats:italic>P. falciparum<\/jats:italic> resistance to generations of antimalarial drugs have prompted the search for new, effective, and safe antimalarial agents. This study aimed at investigating the <jats:italic>in vivo<\/jats:italic> antiplasmodial activity of the 70% hydroethanolic extract and constituents of the stem bark of <jats:italic>Myrianthus libericus<\/jats:italic> based on its ethnomedicinal use as an antimalarial agent. The antiplasmodial activity was assessed in Swiss albino mice employing the 4\u2010day suppressive and Rane\u2019s tests. MLB significantly (<jats:italic>p<\/jats:italic> &lt; 0.0001) suppressed parasitaemia by 52.26%, 65.40%, and 77.11% at 50, 100, and 200\u2009mg\u00b7kg<jats:sup>\u22121<\/jats:sup> doses, respectively, in the 4\u2010day suppressive test. In Rane\u2019s test, the highest parasitaemia suppression of 72.50% was recorded at a dose of 200\u2009mg\u00b7kg<jats:sup>\u22121<\/jats:sup> of the extract. Fractionation of the bioactive ethyl acetate fraction by solvent\u2010solvent partitioning and column chromatography led to the isolation of friedelan\u20103\u2010one and stigmasterol being reported for the first time from this species. The compounds demonstrated remarkable antiplasmodial activity by suppressing parasitaemia by 65\u201372% in the suppressive test and 61\u201370% in the curative test at doses of 10\u201330\u2009mg\u00b7kg<jats:sup>\u22121<\/jats:sup>. Both the extract and the isolated compounds significantly prolonged the survival time of infected mice and averted the cardinal signs associated with <jats:italic>P. berghei<\/jats:italic>\u2010induced malaria including weight loss, hypothermia, and haemolysis. The results obtained confirm the prospect of <jats:italic>M. libericus<\/jats:italic> as an important source of new antimalarial compounds and justifies its folkloric use as an antimalarial agent.<\/jats:p>","DOI":"10.1155\/2020\/8703197","type":"journal-article","created":{"date-parts":[[2020,4,21]],"date-time":"2020-04-21T23:31:57Z","timestamp":1587511917000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<i>In Vivo<\/i> Antiplasmodial Activity of Different Solvent Extracts of <i>Myrianthus libericus<\/i> Stem Bark and Its Constituents in <i>Plasmodium berghei<\/i>\u2010Infected Mice"],"prefix":"10.1155","volume":"2020","author":[{"given":"Michael Kwesi","family":"Baah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9251-4620","authenticated-orcid":false,"given":"Abraham Yeboah","family":"Mensah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5406-9650","authenticated-orcid":false,"given":"Evelyn","family":"Asante-Kwatia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isaac Kingsley","family":"Amponsah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0548-2400","authenticated-orcid":false,"given":"Arnold Donkor","family":"Forkuo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9051-4758","authenticated-orcid":false,"given":"Benjamin Kingsley","family":"Harley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silas","family":"Adjei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,21]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.30875\/f309483f-en"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.11.006"},{"key":"e_1_2_9_3_2","first-page":"187","article-title":"Approaches, challenges and prospects of antimalarial drug discovery from plant sources","author":"Ezenyi I. C.","year":"2016","journal-title":"Current Topics in Malaria"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40794-016-0027-8"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/731906"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3121050"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-8741(03)00141-7"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1155165"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/medicines6010001"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2012.09.028"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-016-5080-8"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1630405"},{"key":"e_1_2_9_13_2","volume-title":"The Useful Plants of West Africa: Families A-D","author":"Burkill H.","year":"1985"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.4314\/jasem.v19i3.28"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.5897\/ajb12.236"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(95)00486-q"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.5897\/jmpr2017.6329"},{"key":"e_1_2_9_18_2","volume-title":"Trease and Evans\u2019 Pharmacognosy E-Book","author":"Evans W. C.","year":"2009"},{"key":"e_1_2_9_19_2","volume-title":"Guidelines for Testing of Chemicals: Guideline 425: Acute Oral Toxicity","author":"Organization of Economic Co-operation and Development (OECD)","year":"2008"},{"key":"e_1_2_9_20_2","first-page":"118","article-title":"In vivo antiplasmodial activities of ethanolic leaf extract and fractions of Hilleria latifolia","volume":"5","author":"Johnson I. S.","year":"2017","journal-title":"Journal of Medicnal Plant Studies"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/4169397"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-016-1529-7"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5212"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.5923\/j.chemistry.20110102.11"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.5897\/ajb2015.15091"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.3329\/icpj.v1i9.11613"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1177\/2050312119849766"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1416"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-8741(01)00270-7"},{"key":"e_1_2_9_30_2","first-page":"62","article-title":"Plasmodium berghei ANKA infection in ICR mice as a model of cerebral malaria","volume":"7","author":"Basir R.","year":"2012","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-018-2509-9"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.05.015"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2015.995632"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2016.108"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2017040"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2005.09.004"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-13-81"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-13-81"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19051382"},{"key":"e_1_2_9_40_2","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.55.464"},{"key":"e_1_2_9_41_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules181012313"},{"key":"e_1_2_9_42_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2003-43212"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/8703197.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/8703197.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/8703197","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T09:07:44Z","timestamp":1723021664000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/8703197"}},"subtitle":[],"editor":[{"given":"Filippo","family":"Fratini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/8703197"],"URL":"https:\/\/doi.org\/10.1155\/2020\/8703197","archive":["Portico"],"relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"value":"1741-427X","type":"print"},{"value":"1741-4288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2019-08-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8703197"}}