{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T23:27:02Z","timestamp":1772062022207,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T00:00:00Z","timestamp":1609286400000},"content-version":"vor","delay-in-days":364,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673803"],"award-info":[{"award-number":["81673803"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>The Tsc1\u2010mTOR signaling pathway is often related to obesity, and epigenetic modification may lead to expression changes of obesity\u2010related gene. Therefore, we aim to investigate the methylation of the Tsc1\u2010mTOR signaling pathway in regulation of obesity susceptibility. Wistar rats were fed a normal diet or a high\u2010fat diet to develop animal models. Protein and mRNA expression levels of Tsc1\u2010mTOR signaling in the hypothalamus were determined by Western blot and quantitative real\u2010time PCR. Methylation of Tsc1 gene promoter was detected by bisulfite genomic sequence. Both mRNA and protein expression levels of Tsc1 in DIO group hypothalamus were lower; mTOR and its downstream targets S6K1, 4EBP1, and S6 protein expression levels were higher than those of the DIO\u2010R group and the chow group. The Tsc1 gene promoter methylation rate in the hypothalamus was 92.05 \u00b1 3.07<jats:italic>%<\/jats:italic> in the DIO group, 87.27 \u00b1 1.91<jats:italic>%<\/jats:italic> in the DIO\u2010R group, and 88.18<jats:italic>%<\/jats:italic> \u00b1 3.20<jats:italic>%<\/jats:italic> in the chow group, respectively, with significantly higher levels in the DIO group. Both the expression levels of Tsc1 gene promoter methylation and Tsc1\u2010mTOR signaling pathway in the hypothalamus of DIO rats and DIO\u2010R rats are different. These findings may shed light on the potential mechanism for the differentiation of obesity susceptibility.<\/jats:p>","DOI":"10.1155\/2020\/8723869","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T18:31:15Z","timestamp":1609353075000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Methylation of Hypothalamic Tsc1\u2010mTOR Signaling in Regulation of Obesity and Obesity 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