{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T02:36:11Z","timestamp":1788834971891,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T00:00:00Z","timestamp":1609286400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003705","name":"Korea Institute of Toxicology","doi-asserted-by":"publisher","award":["KK-2007"],"award-info":[{"award-number":["KK-2007"]}],"id":[{"id":"10.13039\/501100003705","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Analytical Methods in Chemistry"],"published-print":{"date-parts":[[2020,12,30]]},"abstract":"<jats:p>Human beings are easily exposed to formaldehyde (FA) in a living environment. Entry of FA into the human body can have adverse effects on human health, depending on the FA concentration. Thus, a quantitative analysis of FA in blood is necessary in order to estimate its effect on the human body. In this study, a simple and rapid analytical method for the quantitation of FA in blood was developed. The total analysis time, including the pretreatment procedure, was less than 20\u2009min. To ensure a stable analysis, blood samples were stabilized using tripotassium ethylenediaminetetraacetic acid solution, and FA was selectively derivatized using 2,4-dinitrophenylhydrazine as pretreatment procedures. The pretreated samples were analyzed using a high-performance liquid chromatography-UV system, which is the most common choice for analyzing small-molecule aldehydes like formaldehyde. Verification of the pretreatment methods (stabilization and derivatization) using FA standards confirmed that the pretreatment methods are highly reliable in the calibration range 0.012\u20135.761\u2009ng\u2009\u03bcL\u20131 (slope\u2009=\u2009684,898, R2\u2009=\u20090.9998, and limit of detection\u2009=\u20090.251\u2009pg\u00b7\u03bcL\u20131). Analysis of FA in the blood samples of a Yucatan minipig using the new method revealed an average FA concentration of 1.98\u2009\u00b1\u20090.34\u2009ng\u2009\u03bcL\u20131 (n\u2009=\u20093). Blood samples spiked with FA standards were analyzed, and the FA concentrations were found to be similar to the theoretical concentrations (2.16\u2009\u00b1\u20090.81% difference). The method reported herein can quantitatively analyze FA in blood at a sub-nanogram level within a short period of time and is validated for application in blood analysis.<\/jats:p>","DOI":"10.1155\/2020\/8810726","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T20:50:13Z","timestamp":1609361413000},"page":"1-9","source":"Crossref","is-referenced-by-count":7,"title":["Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5299-3882","authenticated-orcid":true,"given":"Yong-Hyun","family":"Kim","sequence":"first","affiliation":[{"name":"Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongeup 56212, Republic of Korea"},{"name":"Human and Environmental Toxicology, University of Science and Technology, Daejeon 34113, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeongsik","family":"Park","sequence":"additional","affiliation":[{"name":"Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongeup 56212, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1159\/000236876"},{"key":"2","first-page":"91","article-title":"Formaldehyde exposure enhances inhalative allergic sensitization in the guinea pig","volume":"51","author":"F. 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