{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T02:44:12Z","timestamp":1771296252729,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T00:00:00Z","timestamp":1607904000000},"content-version":"vor","delay-in-days":348,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY19H030013"],"award-info":[{"award-number":["LY19H030013"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY15H030011"],"award-info":[{"award-number":["LY15H030011"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>This study aimed to investigate the effect of bile duct\u2010targeting lecithins\u2010 (PC\u2010) coupled decorin (DCN) (PC\u2010DCN) nanoliposomes against liver fibrosis in vitro and in vivo. We prepared PC\u2010DCN nanoliposomes by using rat astrocytes, HSC\u2010T6, to verify the antifibrosis effect of PC\u2010DCN in vitro. First, we established a rat model of carbon tetrachloride\u2010induced fibrosis. PC\u2010DCN nanoliposomes were then injected into fibrotic rats via the portal vein or bile duct. The EdU assay was performed to analyze cell proliferation. Immunofluorescence staining was used to detect <jats:italic>\u03b1<\/jats:italic>\u2010smooth muscle actin (<jats:italic>\u03b1<\/jats:italic>\u2010SMA) expression. Western blot was performed to examine the expression of <jats:italic>\u03b1<\/jats:italic>\u2010SMA, collagen type I alpha 1 (COL1A1), and transforming growth factor\u2010<jats:italic>\u03b2<\/jats:italic> (TGF\u2010<jats:italic>\u03b2<\/jats:italic>) protein. The levels of aspartate transaminase (AST), alanine transaminase (ALT), and total bilirubin (TBIL) were examined by enzyme\u2010linked immunosorbent assay (ELISA) analysis. Hematoxylin and eosin (H&amp;E) staining and Masson trichrome staining were used to determine liver tissue lesions and liver fibrosis. Compared with TGF\u2010<jats:italic>\u03b2<\/jats:italic> group, PC\u2010DCN treatment could significantly reduce cell proliferation. Western blot analysis indicated that the expression of <jats:italic>\u03b1<\/jats:italic>\u2010SMA, COL1A1, and TGF\u2010<jats:italic>\u03b2<\/jats:italic> was downregulated after treatment with PC\u2010DCN in vitro and in vivo. Immunofluorescence staining confirmed that <jats:italic>\u03b1<\/jats:italic>\u2010SMA expression was reduced by PC\u2010DCN. Furthermore, H&amp;E staining and Masson trichrome staining showed that the administration of PC\u2010DCN nanoliposomes via the bile duct could reduce the extent of liver fibrosis. PCR analysis showed that PC\u2010DCN administration could reduce proinflammatory cytokines IL\u20106, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, and IL\u20101<jats:italic>\u03b2<\/jats:italic> expression via the bile duct. The administration of PC\u2010DCN nanoliposomes also significantly downregulated liver function indicators ALT, AST, and TBIL. The results of our study indicated that PC\u2010DCN could effectively reduce the extent of liver fibrosis.<\/jats:p>","DOI":"10.1155\/2020\/8815904","type":"journal-article","created":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T17:39:17Z","timestamp":1607967557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Effect of Lecithins Coupled Decorin Nanoliposomes on Treatment of Carbon Tetrachloride\u2010Induced Liver 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