{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T19:45:05Z","timestamp":1789674305499,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,9,9]],"date-time":"2020-09-09T00:00:00Z","timestamp":1599609600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Analytical Methods in Chemistry"],"published-print":{"date-parts":[[2020,9,9]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. To establish a method for extraction and determination of antioxidant 264 (2,6-di-tert-butyl-4-methylphenol) in the brominated butyl rubber stopper for injection and migration study in recombinant potent antitumor and antivirus protein injection (Novaferon). <jats:italic>Methods<\/jats:italic>. Dichloromethane-ethanol was adopted as the extraction solvent during the process of reflux extraction of antioxidant 264 in the brominated butyl rubber stopper. High-performance liquid chromatography (HPLC) was used for the determination of the migration of antioxidant 264 to Novaferon. The mobile phase consisted of acetonitrile-water (80\u2009:\u200920, v\/v). The flow rate was 1.5\u2009mL\/min. The detection wavelength was 280\u2009nm. <jats:italic>Results<\/jats:italic>. The linearity range was from 4.003 to 200.150\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.99996), and the average recovery of antioxidant 264 was 97.8%. The applicability of the methodology was good, which can be used for the determination of antioxidant 264. The results indicated that antioxidant 264 was not detected in Novaferon after the accelerated test and three months of long-term test. <jats:italic>Conclusion<\/jats:italic>. The established validated method in this study can be used for the determination of antioxidant 264 in the rubber stopper, and the brominated butyl rubber stopper has good compatibility with Novaferon.<\/jats:p>","DOI":"10.1155\/2020\/8827925","type":"journal-article","created":{"date-parts":[[2020,9,9]],"date-time":"2020-09-09T23:32:11Z","timestamp":1599694331000},"page":"1-6","source":"Crossref","is-referenced-by-count":11,"title":["Determination of Antioxidant 264 in the Butyl Rubber Stopper and the Compatibility with Recombinant Potent Antitumor and Antivirus Protein Injection"],"prefix":"10.1155","volume":"2020","author":[{"given":"Min","family":"Zhao","sequence":"first","affiliation":[{"name":"School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianhui","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shenyang Wellwolf Pharmaceutical Science and Technology Co. Ltd., Shenyang 110022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5330-0689","authenticated-orcid":true,"given":"Longshan","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","first-page":"1353","volume":"50","year":"2015","journal-title":"Chinese the Pharmaceutical Journal"},{"key":"2","first-page":"56","volume":"25","year":"2013","journal-title":"Tianjin Pharmacy"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2006.08.020"},{"key":"4","first-page":"352","volume":"22","year":"2004","journal-title":"Journal of Pharmacy Practice"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1002\/pts.2770020404"},{"key":"6","first-page":"527","volume":"63","year":"2009","journal-title":"PDA Journal of Pharmaceutical Science and Technology"},{"key":"7","first-page":"2112","volume":"37","year":"2017","journal-title":"Chinese Journal of Pharmaceutical Analysis"},{"key":"8","first-page":"8","volume":"29","year":"2017","journal-title":"Strait Pharmaceutical Journal"},{"key":"9","first-page":"1","year":"2016","journal-title":"Beijing University of Chemical Technology"},{"key":"10","first-page":"340","volume":"4","year":"2017","journal-title":"Changshan Kaijiejian Biopharm Research & Development (Hebei) Co., Ltd."},{"key":"11","first-page":"36","volume":"8","year":"2012","journal-title":"Standard Test"},{"key":"12","doi-asserted-by":"crossref","first-page":"2566","DOI":"10.1002\/jps.20984","volume":"96","year":"2007","journal-title":"Journal of Pharmaceutical Sciences"},{"key":"14","first-page":"1366","volume":"46","year":"2015","journal-title":"Chinese Journal of Pharmaceuticals Industry"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.6023\/cjoc201507020"},{"key":"16","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1016\/j.jpba.2003.08.003","volume":"34","year":"2004","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2008.11.021"},{"key":"19","first-page":"381","year":"2015","journal-title":"National Drug Package Materials Standard"},{"key":"20","first-page":"797","volume":"28","year":"2012","journal-title":"The Chinese Journal of Clinical Pharmacology"},{"key":"21","first-page":"1247","volume":"36","year":"2007","journal-title":"Applied Chemical Industry"},{"key":"22","first-page":"436","volume":"29","year":"2016","journal-title":"Heilongjiang Medical Journal"},{"key":"23","first-page":"801","volume":"44","year":"2013","journal-title":"Chinese Journal of Pharmaceuticals"},{"key":"24","year":"2006"},{"key":"26","first-page":"42","volume":"11","year":"2013","journal-title":"The Science and Technology of Rubber"},{"key":"27","first-page":"2231","volume":"36","year":"2016","journal-title":"Chinese Journal of Pharmaceutical Analysis"},{"key":"28","first-page":"1473","volume":"24","year":"2011","journal-title":"Chinese Journal of Biology"},{"key":"29","first-page":"702","volume":"37","year":"2017","journal-title":"Chinese Journal of Pharmaceutical Analysis"},{"key":"30","first-page":"940","volume":"23","year":"2014","journal-title":"Chinese Journal of New Drugs"},{"key":"31","year":"2015"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1080\/10837450.2017.1312442"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2009.03.015"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2017.04.012"}],"container-title":["Journal of Analytical Methods in Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jamc\/2020\/8827925.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jamc\/2020\/8827925.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jamc\/2020\/8827925.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,9]],"date-time":"2020-09-09T23:32:19Z","timestamp":1599694339000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jamc\/2020\/8827925\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,9]]},"references-count":31,"alternative-id":["8827925","8827925"],"URL":"https:\/\/doi.org\/10.1155\/2020\/8827925","relation":{},"ISSN":["2090-8865","2090-8873"],"issn-type":[{"value":"2090-8865","type":"print"},{"value":"2090-8873","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,9]]}}}