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This study is the first to evaluate the effect of a 4-week NAC supply on the antioxidant barrier and oxidative stress in Wistar rats after six weeks of high-fat diet (HFD) intake. Redox biomarkers were evaluated in the parotid (PG) and submandibular (SMG) salivary glands and stimulated whole saliva (SWS), as well as in the plasma and serum. We demonstrated that the activity of salivary peroxidase and superoxide dismutase and total antioxidant capacity were significantly higher in PG, SMG, and SWS of IR rats treated with NAC. It appears that in PG and SMG of rats fed an HFD, N-acetylcysteine supplementation abolishes oxidative modifications to proteins (evidenced by decreased content of advanced oxidation protein products (AOPP) and advanced glycation end products (AGE)). Simultaneously, it does not reverse oxidative modifications of lipids (as seen in increased concentration of 8-isoprostanes and 4-hydroxynonenal vs. the control), although it reduces the peroxidation of salivary lipids in relation to the group fed a high-fat diet alone. NAC administration increased protein levels in PG and SMG but did not affect saliva secretion, which was significantly lower compared to the controls. To sum up, the inclusion of NAC supplementation after six weeks of HFD feeding was effective in improving the general and salivary gland antioxidant status. Nevertheless, NAC did not eliminate salivary oxidative stress and only partially prevented salivary gland dysfunction.<\/jats:p>","DOI":"10.1155\/2020\/8831855","type":"journal-article","created":{"date-parts":[[2020,11,17]],"date-time":"2020-11-17T02:50:23Z","timestamp":1605581423000},"page":"1-15","source":"Crossref","is-referenced-by-count":12,"title":["NAC Supplementation of Hyperglycemic Rats Prevents the Development of Insulin Resistance and Improves Antioxidant Status but Only Alleviates General and Salivary Gland Oxidative Stress"],"prefix":"10.1155","volume":"2020","author":[{"given":"Anna","family":"Zalewska","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Dentistry, Medical University of Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Zi\u0119ba","sequence":"additional","affiliation":[{"name":"Student of the Faculty of Medicine with the Division of Dentistry at the Medical University of Bialystok, Poland; Students Scientific Club \u201cBiochemistry of Civilization Diseases\u201d at the Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Kostecka-Socho\u0144","sequence":"additional","affiliation":[{"name":"Conservative Dentistry Department, Medical University of Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Kossakowska","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Dentistry, Medical University of Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u0142gorzata","family":"\u017bendzian-Piotrowska","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Matczuk","sequence":"additional","affiliation":[{"name":"County Veterinary Inspection, Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5609-3187","authenticated-orcid":true,"given":"Mateusz","family":"Maciejczyk","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Epidemiology and Ergonomics, Medical University of Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8122197"},{"issue":"2","key":"2","first-page":"81","article-title":"The role of dietary antioxidants on oxidative stress in diabetic nephropathy","volume":"14","author":"F. 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