{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T17:29:18Z","timestamp":1792171758799,"version":"4.4.0"},"reference-count":61,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T00:00:00Z","timestamp":1597449600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009402","name":"Mekelle University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009402","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2020,8,15]]},"abstract":"<jats:p>Antibiotic-resistant<jats:italic>Staphylococcus aureus<\/jats:italic>isolates pose a severe challenge to both veterinary and health professions and dairy cattle producers. Cross-sectional study was conducted from November 2014 to May 2015 to isolate and identify<jats:italic>S. aureus<\/jats:italic>from mastitic cows\u2019 milk and estimate the occurrence of MRSA in the dairy farms of Shire. Physical examination and California mastitis test were performed on a total of 220 dairy cows. Bacteriological isolation and identification and antibiogram testing were performed. Furthermore, multiplex polymerase chain reaction (PCR) was done for the detection of<jats:italic>mec A<\/jats:italic>and<jats:italic>fem A<\/jats:italic>genes. Out of the 220 dairy cows, 64 (29.09%) were positive for bovine mastitis, and of these, 32.81% were coagulase-positive<jats:italic>S. aureus<\/jats:italic>(CoPS). Antibiogram test results showed 100% of the isolates were resistant to penicillin G, nalidixic acid, and ampicillin, and 33.33% of the CoPS showed resistance to oxacillin (phenotypically MRSA positive). But 38.09% of the CoPS were found to be resistant and susceptible to vancomycin. PCR amplification of the seven phenotypically MRSA isolates revealed that 42.9% and 71.4% of them were found to carry<jats:italic>fem A<\/jats:italic>and<jats:italic>mec A<\/jats:italic>genes, respectively. The current study revealed the existence of alarming level of CoPS and development of multidrug resistance.<\/jats:p>","DOI":"10.1155\/2020\/8833973","type":"journal-article","created":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T23:30:15Z","timestamp":1597534215000},"page":"1-7","source":"Crossref","is-referenced-by-count":25,"title":["Isolation and Identification of Methicillin-Resistant<i>Staphylococcus aureus<\/i>(MRSA) from Milk in Shire Dairy Farms, Tigray, Ethiopia"],"prefix":"10.1155","volume":"2020","author":[{"given":"Weldemelak","family":"Girmay","sequence":"first","affiliation":[{"name":"College of Veterinary Sciences, Mekelle University, Mekelle, Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1201-2263","authenticated-orcid":true,"given":"Getachew","family":"Gugsa","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Wollo University, Dessie, 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