{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:19:06Z","timestamp":1777443546411,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T00:00:00Z","timestamp":1607040000000},"content-version":"vor","delay-in-days":338,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY19H230001"],"award-info":[{"award-number":["LY19H230001"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>In this paper, a UPLC\u2010MS\/MS method was developed for the determination of ropivacaine and its metabolite 3\u2010hydroxy ropivacaine in cerebrospinal fluid. The cerebrospinal fluid was processed by ethyl acetate liquid\u2010liquid extraction. The multiple reaction monitoring (MRM) mode was used for quantitative analysis by monitoring the transitions of m\/z 275.3 \u2192 126.2 for ropivacaine, m\/z 291.0 \u2192 126.0 for 3\u2010hydroxy ropivacaine, and m\/z 290.2 \u2192 198.2 for the internal standard. Standard curves for ropivacaine and 3\u2010hydroxy ropivacaine in cerebrospinal fluid were conducted over the concentration range of 0.2\u20132000\u2009ng\/mL, demonstrating excellent linearity, and the lower limit of quantification was 0.2\u2009ng\/mL. The intraday precision of ropivacaine and 3\u2010hydroxy ropivacaine was less than 11%, while the interday precision was less than 7%. The accuracy ranged between 87% and 107%, the average extraction efficiency was higher than 79%, and the matrix effect was between 89% and 98%. The developed method was then applied to a case of suspected poisoning of ropivacaine.<\/jats:p>","DOI":"10.1155\/2020\/8844866","type":"journal-article","created":{"date-parts":[[2020,12,4]],"date-time":"2020-12-04T23:21:32Z","timestamp":1607124092000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Simultaneous Determination of Ropivacaine and 3\u2010Hydroxy Ropivacaine in Cerebrospinal Fluid by UPLC\u2010MS\/MS"],"prefix":"10.1155","volume":"2020","author":[{"given":"Siyuan","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianshe","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9821-3770","authenticated-orcid":false,"given":"Xianqin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2755-1422","authenticated-orcid":false,"given":"Quan","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,12,4]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.4103\/0019-5049.79875"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-200565180-00013"},{"key":"e_1_2_8_3_2","first-page":"251","article-title":"Intrathecal use of ropivacaine: a review","volume":"55","author":"Wille M.","year":"2004","journal-title":"Acta Anaesthesiologica Belgica"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0b013e3181e3a08e"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmig.2008.05.012"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1586\/14737175.4.5.781"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.2147\/jpr.s247158"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.2147\/jpr.s229523"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12871-019-0855-y"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007691-200104000-00015"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/47.3.231"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2018-1298"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200600087"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-005-0042-5"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2009.11.028"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.2220\/biomedres.30.319"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.11.035"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.731"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1570-0232(02)00280-5"},{"key":"e_1_2_8_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-4347(00)00346-7"},{"key":"e_1_2_8_21_2","unstructured":"FDA Guidance for Industry Analytical Procedures and Methods Validation for Drugs and Biologics 2014 https:\/\/www.fdanews.com\/ext\/resources\/files\/02\/02-19-14-Guidance.pdf."},{"key":"e_1_2_8_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2017.06.018"},{"key":"e_1_2_8_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.12.006"},{"key":"e_1_2_8_24_2","doi-asserted-by":"publisher","DOI":"10.1556\/1326.2019.00586"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.2174\/1573412916666191022161501"},{"key":"e_1_2_8_26_2","first-page":"1105","article-title":"Pharmacokinetics of talatisamine in rat plasma by UPLC-MS\/MS","volume":"39","author":"Lin G. 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