{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T13:10:52Z","timestamp":1779369052298,"version":"3.53.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,29]],"date-time":"2020-11-29T00:00:00Z","timestamp":1606608000000},"content-version":"vor","delay-in-days":333,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF-2019R1G1A1100391"],"award-info":[{"award-number":["NRF-2019R1G1A1100391"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>The global obesity epidemic has nearly doubled since 1980, and this increasing prevalence is threatening public health. It has been reported that natural products could contain potential functional ingredients that may assist in preventing obesity. Bojungchiseub\u2010tang (BJT), mentioned in the <jats:italic>Donguibogam<\/jats:italic> as an herbal medication for the treatment of edema, a symptom of obesity, consists of eleven medicinal herbs. However, the pharmacological activity of BJT has not been investigated. The present study was designed to investigate the putative effect of BJT on the adipogenesis of 3T3\u2010L1 cells and the weight gain of high\u2010fat diet (HFD\u2010) fed C57BL\/6 mice. Oil Red O staining was conducted to examine the amount of lipids in 3T3\u2010L1 adipocytes. Male C57BL\/6 mice were divided into three groups: standard diet group (control, CON), 45% HFD group (HFD), and HFD supplemented with 10% of BJT (BJT). The expression levels of genes and proteins related to adipogenesis in cells, WAT, and liver were analyzed by quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) and western blot, respectively. We found that BJT treatment significantly decreased the protein and mRNA levels of peroxisome proliferator\u2010activated receptor <jats:italic>\u03b3<\/jats:italic> (PPAR<jats:italic>\u03b3<\/jats:italic>), CCAAT\/enhancer\u2010binding protein <jats:italic>\u03b1<\/jats:italic> (C\/EBP<jats:italic>\u03b1<\/jats:italic>), and sterol regulatory element\u2010binding protein 1 (SREBP1) in a dose\u2010dependent manner in differentiated 3T3\u2010L1 cells. Similar to the results of the <jats:italic>in vitro<\/jats:italic> experiment, BJT suppressed HFD\u2010induced weight gain in an obese mouse model. In addition, BJT effectively reduced the HFD\u2010induced epididymal adipose tissue weight\/body weight index. BJT also downregulated the mRNA levels of <jats:italic>PPAR\u03b3<\/jats:italic>, <jats:italic>C\/EBP\u03b1<\/jats:italic>, and <jats:italic>SREBP1<\/jats:italic> in the epididymal adipose and liver tissue of HFD\u2010fed obese mice. These findings suggest that BJT induces weight loss by affecting adipogenic transcription factors.<\/jats:p>","DOI":"10.1155\/2020\/8851010","type":"journal-article","created":{"date-parts":[[2020,11,29]],"date-time":"2020-11-29T16:35:07Z","timestamp":1606667707000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Pharmacological Properties of a Traditional Korean Formula Bojungchiseup\u2010tang on 3T3\u2010L1 Preadipocytes and High\u2010Fat Diet\u2010Induced Obesity Mouse Model"],"prefix":"10.1155","volume":"2020","author":[{"given":"Yea-Jin","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Wook","family":"Seo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae-Young","family":"Gil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Divina 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