{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T19:52:55Z","timestamp":1791575575850,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T00:00:00Z","timestamp":1609286400000},"content-version":"vor","delay-in-days":364,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008257","name":"Tarbiat Modares University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008257","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    The aim of this study was to evaluate the efficacy of selenium nanoparticle (an immune booster) and naloxone (an opioid receptor antagonist) as a new adjuvant in increasing immune responses against killed whole\u2010cell\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    in a mouse cholera model. The Se NPs were synthesized and characterized by UV\u2010visible, DLS, and zeta potential analysis. The SEM image confirmed the uniformity of spherical morphology of nanoparticle shape with 34\u2009nm in size. The concentration of the Se NPs was calculated as 0.654\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/ml in the ICP method. The cytotoxic activity of Se NPs on Caco\u20102 cells was assessed by the MTT assay and revealed 82.05% viability of cells after 24\u2009h exposure with 100\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/ml of Se NPs. Female BALB\/C mice were orally immunized three times on days 0, 14, and 28, and challenge experiments were performed on immunized neonates with toxigenic\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    . Administration of Se NP diet led to significant increase in\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    \u2010specific IgG and IgA responses in serum and saliva and caused protective immunity and 83.3% survival in challenge experiment against 1 LD50\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    in a group receiving diet of Se NPs compared with other groups including Dukoral vaccine. The IL\u20104 and IL\u20105 were significantly increased in response to WC+daily diet of Se NPs with or without naloxone. Naloxone proved no effect on IL\u20104 and IL\u20105 increase and is proposed as null in the cytokine and antibody production process. These results reveal that daily diet of Se NPs could efficiently induce immune cell effectors in both humoral and mucosal levels.\n                  <\/jats:p>","DOI":"10.1155\/2020\/8874288","type":"journal-article","created":{"date-parts":[[2020,12,30]],"date-time":"2020-12-30T15:35:10Z","timestamp":1609342510000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Selenium Nanoparticles Induce Potent Protective Immune Responses against\n                    <i>Vibrio cholerae<\/i>\n                    WC Vaccine in a Mouse Model"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4209-6624","authenticated-orcid":false,"given":"Zahra","family":"Raahati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7523-7905","authenticated-orcid":false,"given":"Bita","family":"Bakhshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5404-9190","authenticated-orcid":false,"given":"Shahin","family":"Najar-peerayeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,30]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(11)60149-1"},{"key":"e_1_2_9_2_2","article-title":"Cholera, 2016","volume":"92","author":"Organization WH","year":"2017","journal-title":"Weekly Epidemiological Record= Relev\u00e9 \u00e9pid\u00e9miologique hebdomadaire"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3251"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.10.008"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(79)90119-6"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07084"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2014.82"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0742-8413(90)90007-V"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2007.02.005"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.5.1463S"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0753-3322(03)00035-0"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4145"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2010.02.001"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxn139"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01197-08"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1039\/c2an35433k"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2017.01.018"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05689-11"},{"key":"e_1_2_9_19_2","unstructured":"IoLARCoC.andAnimalsU. 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