{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:01:17Z","timestamp":1787587277470,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,24]],"date-time":"2020-10-24T00:00:00Z","timestamp":1603497600000},"content-version":"vor","delay-in-days":297,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011665","name":"Deanship of Scientific Research, King Saud University","doi-asserted-by":"publisher","award":["RG-1441-486"],"award-info":[{"award-number":["RG-1441-486"]}],"id":[{"id":"10.13039\/501100011665","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Current treatments for complex diseases have remarkable side effects that negatively impact patients\u2019 quality of life. Thus, natural compounds with fewer side effects represent a promising source for safe drugs. The genus <jats:italic>Senecio<\/jats:italic> is widely used in folk medicine due to its various pharmacological properties. In the present study, the total phenolic content of <jats:italic>Senecio glaucus<\/jats:italic>, which is grown in Saudi Arabia, was assessed using the Folin\u2010Ciocalteau colorimetric method. Scavenging DPPH and ABTS assays were utilized to determine the antioxidant properties of <jats:italic>S. glaucus<\/jats:italic> fractions, and MTT assay was used to screen the cytotoxic activity of <jats:italic>S. glaucus<\/jats:italic> against various cancer cells. In addition, HPLC\u2010UV was utilized to detect the presence of two phenolic acids, namely, vanillic acid (VA) and gallic acid (GA). Among all fractions tested, <jats:italic>S. glaucus<\/jats:italic> chloroform fraction (SGCF) yielded the highest value (125.3\u2009mg\u00b7GA\/g) in terms of total phenolic content. SGCF also exhibited the highest scavenging activities (76.7 and 74.1%) on both DPPH and ABTS assays, respectively. Similarly, SGCF also possessed the most potent cytotoxic activity against the MCF\u20107 cell line, with an IC<jats:sub>50<\/jats:sub> value of 41.8\u2009<jats:italic>\u03bc<\/jats:italic>g\/ml. The validated HPLC method confirmed the presence of VA (4.8\u2009<jats:italic>\u03bc<\/jats:italic>g\/mg DW) and GA (3.9\u2009<jats:italic>\u03bc<\/jats:italic>g\/mg DW) in SGCF. Overall, our data show that <jats:italic>S. glaucus<\/jats:italic> had antioxidant and cytotoxic properties. A developed validated HPLC method which could be helpful for quantifying phenolic compounds in <jats:italic>S. glaucus<\/jats:italic> was established.<\/jats:p>","DOI":"10.1155\/2020\/8875430","type":"journal-article","created":{"date-parts":[[2020,10,24]],"date-time":"2020-10-24T18:35:10Z","timestamp":1603564510000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["In Vitro Antioxidant, Cytotoxic Activities, and Phenolic Profile of <i>Senecio glaucus<\/i> from Saudi Arabia"],"prefix":"10.1155","volume":"2020","author":[{"given":"Ali S.","family":"Alqahtani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rashed N.","family":"Herqash","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar M.","family":"Noman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6496-7822","authenticated-orcid":false,"given":"Fahd A.","family":"Nasr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouf","family":"Alyhya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shamsa H.","family":"Anazi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Farooq","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2860-467X","authenticated-orcid":false,"given":"Riaz","family":"Ullah","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,24]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24020293"},{"key":"e_1_2_9_2_2","first-page":"1","article-title":"Phenolic compounds: functional properties, impact of processing and bioavailability","author":"Minatel I. 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