{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T21:31:46Z","timestamp":1785879106466,"version":"3.56.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T00:00:00Z","timestamp":1596240000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["17ZR1404600"],"award-info":[{"award-number":["17ZR1404600"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["81230019"],"award-info":[{"award-number":["81230019"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["17ZR1404600"],"award-info":[{"award-number":["17ZR1404600"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81230019"],"award-info":[{"award-number":["81230019"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Neural Plasticity"],"published-print":{"date-parts":[[2020,8,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. The precise mechanisms of nerve regeneration remain unclear. The potential of facial nerve regeneration and probable mechanisms involved following chronic facial nerve injury should be further studied. <jats:italic>Methods<\/jats:italic>. Adult male Wistar rats were used to model either (i) facial nerve injury (axotomy) or (ii) reinjury (chronic axotomy followed by a second axotomy within 5 months). The rats were housed in the animal facility of the Eye and ENT Hospital of Shanghai Medical School, Fudan University (Shanghai, China). Expression of Shh (sonic hedgehog) and growth-associated protein 43 (GAP43, a neuronal marker) was detected in bilateral facial nuclei using reverse transcriptase PCR, western blotting analysis, and immunohistochemistry. The number of surviving motoneurons was quantified, and facial nerve regeneration was examined using transmission electron microscopy. <jats:italic>Results<\/jats:italic>. Reinjury of the facial nerve 12 weeks after the first axotomy resulted in upregulation of GAP43 mRNA and protein expression in neurons ipsilateral to the axotomy; immunohistochemistry revealed that Shh expression was higher compared with control side facial nuclei at the same time point. GAP43 expression subsequently decreased. <jats:italic>Conclusion<\/jats:italic>. The greatest regeneration potential of the facial nerve occurred within 5 months following chronic axotomy in rats, and regeneration may involve the Shh signaling pathway.<\/jats:p>","DOI":"10.1155\/2020\/8884511","type":"journal-article","created":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T23:35:57Z","timestamp":1596324957000},"page":"1-9","source":"Crossref","is-referenced-by-count":10,"title":["The Regenerative Potential of Facial Nerve Motoneurons following Chronic Axotomy in Rats"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9784-0676","authenticated-orcid":true,"given":"Yusu","family":"Ni","sequence":"first","affiliation":[{"name":"Otology and Skull Base Surgery Department, Eye and ENT Hospital of Shanghai Medical School, Fudan University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diyan","family":"Chen","sequence":"additional","affiliation":[{"name":"Otology and Skull Base Surgery Department, Eye and ENT Hospital of Shanghai Medical School, Fudan University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, Shanghai Xin Shi Jie Eye Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danhong","family":"Qiu","sequence":"additional","affiliation":[{"name":"Otolaryngology Department, Pudong Hospital, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Li","sequence":"additional","affiliation":[{"name":"Central Laboratory, Eye and ENT Hospital of Shanghai Medical School, Fudan University, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5338-6910","authenticated-orcid":true,"given":"Huawei","family":"Li","sequence":"additional","affiliation":[{"name":"Otology and Skull Base Surgery Department, Eye and ENT Hospital of Shanghai Medical School, Fudan University, China"},{"name":"ENT Institute and Otorhinolaryngology Department of Eye & ENT Hospital, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science, Fudan University, Shanghai 200031, China"},{"name":"Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China"},{"name":"NHC Key Laboratory of Hearing Medicine (Fudan University), Shanghai 200031, China"},{"name":"The Institutes of Brain Science and the Collaborative Innovation Center for Brain Science, Fudan University, Shanghai 200032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","year":"2004"},{"key":"2","year":"2008"},{"key":"3","year":"2015"},{"key":"4","year":"2018"},{"key":"5","year":"2000"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2013.11.006"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2014.10.022"},{"issue":"96","key":"8","volume":"23","year":"2015","journal-title":"Journal of Visualized Experiments"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24123"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1007\/s00106-007-1610-0"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1097\/MAO.0000000000000760"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.4103\/0970-0358.155265"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1002\/micr.30580"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.4103\/1735-3327.182181"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.2972"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2016.02.020"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2011.03.032"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.reth.2019.08.004"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2018.12.006"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12665"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1002\/micr.30389"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1177\/0963689719854446"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2019.06.008"},{"issue":"5","key":"24","first-page":"490","volume":"30","year":"2015","journal-title":"Neurorehabilitation and Neural Repair"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1002\/ar.24127"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1080\/03091902.2019.1637470"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90397-6"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1995.1011"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1126\/science.1058624"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1741-4520.2012.00368.x"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1784-04.2004"},{"issue":"12","key":"32","first-page":"1341","volume":"59","year":"2007","journal-title":"Brain and Nerve"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1210\/endo.142.2.7922"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2004.04.010"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-15-05587.2000"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-11-j0004.1999"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4547(20001015)62:2<273::AID-JNR11>3.0.CO;2-C"}],"container-title":["Neural Plasticity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/np\/2020\/8884511.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/np\/2020\/8884511.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/np\/2020\/8884511.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,1]],"date-time":"2020-08-01T23:36:00Z","timestamp":1596324960000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/np\/2020\/8884511\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,1]]},"references-count":37,"alternative-id":["8884511","8884511"],"URL":"https:\/\/doi.org\/10.1155\/2020\/8884511","relation":{},"ISSN":["2090-5904","1687-5443"],"issn-type":[{"value":"2090-5904","type":"print"},{"value":"1687-5443","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,8,1]]}}}