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Furthermore, autophagy can promote the survival of B and T cells, plasma cell differentiation, and antibody production. These results suggest that autophagy may promote the progression of lupus by regulating the survival of autoreactive immune cells. Therefore, we aimed at studying whether suppressing autophagy can modulate lupus progression <jats:italic>in vivo<\/jats:italic>. First, we found that the autophagy levels in splenocytes and lymphocytes of peripheral blood (PB) were elevated and positively correlated with disease severity in lupus\u2010prone mice. The shAtg5\u2010lentivirus, which effectively inhibits autophagy <jats:italic>in vitro<\/jats:italic>, was then injected into the lupus\u2010prone mice. Autophagy levels in lymph node cells and PB lymphocytes were reduced following Atg5 suppression. We also found that lymphadenopathy and the numbers of plasma cells, CD4<jats:sup>-<\/jats:sup>CD8<jats:sup>-<\/jats:sup>, and CD4<jats:sup>+<\/jats:sup> T cells decreased in mice treated with the shAtg5\u2010lentivirus. The mice treated with shAtg5\u2010lentivirus exhibited lower levels of proteinuria, serum anti\u2010dsDNA antibody, B\u2010cell activating factor (BAFF), and glomerular immune complex deposition. Therefore, targeting autophagy to moderate overactivated autophagy in immune cells seems to be a novel strategy for combination therapy of lupus.<\/jats:p>","DOI":"10.1155\/2020\/8959726","type":"journal-article","created":{"date-parts":[[2020,5,2]],"date-time":"2020-05-02T23:30:37Z","timestamp":1588462237000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<i>In Vivo<\/i> Suppression of Autophagy via Lentiviral shRNA Targeting Atg5 Improves Lupus\u2010Like 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