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However, the role of MitoTEMPO on nonalcoholic fatty liver disease (NAFLD) and its possible mechanisms are largely unknown. Here, we investigated the effects of MitoTEMPO on NAFLD using high fat diet\u2010 (HFD\u2010) induced obese mice as animal models. MitoTEMPO was intraperitoneally injected into HFD mice. Liver morphological changes were observed by H&amp;E and Oil Red O staining, and the frequency of MDSCs in peripheral blood was analyzed by flow cytometry. Moreover, real\u2010time quantitative PCR, western blot, and immunohistochemistry were conducted to detect the mRNA and protein expressions in the liver tissues. The results showed that the hepatic steatosis in liver tissues of HFD mice injected with MitoTEMPO was significantly ameliorated. Additionally, MitoTEMPO reduced the frequency of CD11b<jats:sup>+<\/jats:sup>Gr\u20101<jats:sup>+<\/jats:sup> MDSCs in peripheral circulation and decreased Gr\u20101<jats:sup>+<\/jats:sup> cell accumulation in the livers. Further studies demonstrated that MitoTEMPO administration suppressed the mRNA and protein expressions of MDSC\u2010associated proinflammatory mediators, such as monocyte chemoattractant protein\u20101 (MCP\u20101), S100 calcium\u2010binding protein A8 (S100A8), and S100 calcium\u2010binding protein A9 (S100A9). Our results suggest that MitoTEMPO appears to be a potential chemical compound affecting certain immune cells and further ameliorates inflammation in obese\u2010associated NAFLD.<\/jats:p>","DOI":"10.1155\/2020\/9329427","type":"journal-article","created":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T00:44:13Z","timestamp":1596156253000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Protective Effects of MitoTEMPO on Nonalcoholic Fatty Liver Disease via Regulating Myeloid\u2010Derived Suppressor Cells and Inflammation in 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