{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T11:23:28Z","timestamp":1775820208010,"version":"3.50.1"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"vor","delay-in-days":238,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2018GXNSFBA050068"],"award-info":[{"award-number":["2018GXNSFBA050068"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. To explore the possible role of miR\u2010499a\u20103p in the function of primary human umbilical vein endothelial cells (HUVECs) and the expression of ADAM10 in primary HUVEC. <jats:italic>Method<\/jats:italic>. miR\u2010499a\u20103p was first transfected into primary HUVECs via lentivirus vector. The viability, proliferation, and migration of stable transfected primary HUVEC were then determined by flow cytometry, CCK8 assays, scratch tests, and Transwell tests. The transcription of miR\u2010499a\u20103p and ADAM10 was examined by reverse transcription\u2010polymerase chain reaction (RT\u2010PCR), and the expression of ADAM10 was examined by Western blot (WB). <jats:italic>Results<\/jats:italic>. After transfection, miR\u2010499a\u20103p transcription was significantly increased (<jats:italic>P<\/jats:italic> &lt; 0.01), compared to the blank and nonspecific control (NC) groups, while both ADAM10 transcription and expression were significantly decreased (<jats:italic>P<\/jats:italic> &lt; 0.05). In contrast, in the inhibitors group, miR\u2010499a\u20103p transcription was significantly reduced (<jats:italic>P<\/jats:italic> &lt; 0.05) whereas both ADAM10 transcription and expression were significantly increased (<jats:italic>P<\/jats:italic> &lt; 0.05). The viability, proliferation, and migration of primary HUVECs were significantly impaired (<jats:italic>P<\/jats:italic> &lt; 0.05) by the transfection of miR\u2010499a\u20103p but enhanced by miR\u2010499a\u20103p inhibitors (<jats:italic>P<\/jats:italic> &lt; 0.05). <jats:italic>Conclusions<\/jats:italic>. Upregulation of miR\u2010499a\u20103p transcription will inhibit the expression of ADAM10 in HUVECs; cell migration and proliferation, however, promote apoptosis. And reverse effects were established by downregulation of miR\u2010499a\u20103p transcription. All these effects may be achieved by regulating the transcription and expression of ADAM10. These results combined suggested that miR\u2010499a\u20103p may affect the proliferation, migration, and apoptosis of endothelial cells and regulate AS by regulating ADAM10. miR\u2010499a\u20103p may become a candidate biomarker for the diagnosis of unstable angina pectoris (UA).<\/jats:p>","DOI":"10.1155\/2020\/9372961","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T23:38:51Z","timestamp":1598485131000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Lentivirus\u2010Mediated miR\u2010499a\u20103p on Human Umbilical Vein Endothelial 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