{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T17:53:11Z","timestamp":1762624391644,"version":"3.40.5"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,5,14]],"date-time":"2020-05-14T00:00:00Z","timestamp":1589414400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The Ministry of Education, Science, Research and Sport of the Slovak Republic","award":["VEGA 1\/0212\/17"],"award-info":[{"award-number":["VEGA 1\/0212\/17"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2020,5,14]]},"abstract":"<jats:p><jats:italic>Background.<\/jats:italic> Sarcoidosis and hypersensitivity pneumonitis (HP) are immunologically mediated processes caused by hypersensitivity reaction accompanied by similar features including lymphocytic alveolitis and granuloma formation. Recent studies describe the role of TREM receptors in T cell activation, differentiation, and granuloma formation. Alveolar macrophages activation via TREM receptors may be the key factor mediating subsequent immune response. The aim of the study was to analyse TREM-1 and TREM-2 expression to identify further molecular mechanisms participating in the immunopathogenesis of sarcoidosis and HP. <jats:italic>Methods<\/jats:italic>. Flow cytometry was performed to analyse TREM-1 and TREM-2 expression on CD14<jats:sup>+<\/jats:sup> cells in bronchoalveolar lavage fluid from patients having sarcoidosis or HP and a control group. <jats:italic>Results<\/jats:italic>. The study proved increased TREM-1 expression on alveolar macrophages in pulmonary sarcoidosis and diminished TREM-1 expression in HP-Sarcoidosis: median: 76.7; HP: median: 29.9; control: median: 53.3, (sarcoidosis versus HP: <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>; sarcoidosis versus control: <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). TREM-2 expression was increased in both, sarcoidosis and HP-sarcoidosis: median: 34.79; HP: median: 36.00; control: median: 12.98, (sarcoidosis versus control: <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>; HP versus control: <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). Correlation analysis showed negative correlation between TREM-1 and total number of CD8<jats:sup>+<\/jats:sup> cytotoxic T cells. In sarcoidosis TREM-1 expression decreased with changes of HRCT image, decrease in CD4\/CD8 ratio and decrease in DLCO. <jats:italic>Conclusions<\/jats:italic>. Differences in TREM receptor expression in sarcoidosis (increase in TREM-1 and TREM-2) and HP (increase in TREM-2) and correlation analysis suggests that activation via TREM may participate in typical immunological characteristics of sarcoidosis and HP.<\/jats:p>","DOI":"10.1155\/2020\/9501617","type":"journal-article","created":{"date-parts":[[2020,5,15]],"date-time":"2020-05-15T00:39:31Z","timestamp":1589503171000},"page":"1-9","source":"Crossref","is-referenced-by-count":5,"title":["TREM-1 and TREM-2 Expression on CD14<sup>+<\/sup> Cells in Bronchoalveolar Lavage Fluid in Pulmonary Sarcoidosis and Hypersensitivity Pneumonitis in the Context of T Cell Immune Response"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5516-9845","authenticated-orcid":true,"given":"M.","family":"Suchankova","sequence":"first","affiliation":[{"name":"Institute of Immunology, Faculty of Medicine Comenius University, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Urban","sequence":"additional","affiliation":[{"name":"National Institute for Tuberculosis, Lung Diseases and Thoracic Surgery, Vysne Hagy, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ganovska","sequence":"additional","affiliation":[{"name":"National Institute for Tuberculosis, Lung Diseases and Thoracic Surgery, Vysne Hagy, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Tibenska","sequence":"additional","affiliation":[{"name":"Medirex Ltd., Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Szaboova","sequence":"additional","affiliation":[{"name":"Medirex Ltd., Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Tedlova","sequence":"additional","affiliation":[{"name":"Department of Pneumology and Phthisiology, Faculty of Medicine Comenius University and University Hospital, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Sandor","sequence":"additional","affiliation":[{"name":"Department of Pneumology and Phthisiology, Faculty of Medicine Comenius University and University Hospital, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Majer","sequence":"additional","affiliation":[{"name":"Department of Pneumology and Phthisiology, Faculty of Medicine Comenius University and University Hospital, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bobovcak","sequence":"additional","affiliation":[{"name":"Beckman Coulter Ltd., Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Jonner","sequence":"additional","affiliation":[{"name":"National Institute for Tuberculosis, Lung Diseases and Thoracic Surgery, Vysne Hagy, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Konig","sequence":"additional","affiliation":[{"name":"University of Economics in Bratislava, Faculty of Economic Informatics, Department of Operations Research and Econometrics, Bratislava, Slovakia"},{"name":"Institute of Economic Research of Slovak Academy of Sciences, Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4742-3505","authenticated-orcid":true,"given":"M.","family":"Bucova","sequence":"additional","affiliation":[{"name":"Institute of Immunology, Faculty of Medicine Comenius University, Bratislava, 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