{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:20:11Z","timestamp":1790803211284,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T00:00:00Z","timestamp":1603324800000},"content-version":"vor","delay-in-days":295,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20190904"],"award-info":[{"award-number":["BK20190904"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    The purpose of this study was to investigate the effect of knockdown of the yes\u2010associated protein (YAP) and transcriptional coactivator with PDZ\u2010binding motif (TAZ) on the migration and invasion of the rheumatoid arthritis fibroblast\u2010like synoviocytes (RA\u2010FLS) and to preliminarily elucidate the mechanisms between YAP\/TAZ and autophagy in the migration and invasion of RA\u2010FLS. RA\u2010FLS stable knockdown of YAP or TAZ was successfully established by using lentiviral\u2010mediated gene knockdown techniques. Wound healing assay and Transwell assay were used to evaluate the effect of knockdown of YAP or TAZ on the migration and invasion of RA\u2010FLS. Reverse transcription quantitative real\u2010time polymerase chain reaction (RT\u2010qPCR) and western blotting assays were performed to examine the expression of indicated genes. The results showed that YAP and TAZ were upregulated in RA\u2010FLS, and knockdown of YAP or TAZ inhibited the migration and invasion, reduced the expression of N\u2010cadherin and Vimentin, and increased the accumulation of E\u2010cadherin and\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010catenin in RA\u2010FLS. Our results also demonstrated that knockdown of YAP or TAZ promoted autophagy which increased the accumulation of LC3B\u2010II and ULK1 and decreased the amount of SQSTM1\/p62 in RA\u2010FLS. Furthermore, our data displayed that inhibition of autophagy either with 3\u2010MA or CQ can partially reverse the decrease of migration and invasion induced by YAP and TAZ knockdown in RA\u2010FLS. Our experiments preliminarily revealed that YAP\/TAZ and autophagy play important roles in the migration and invasion of RA\u2010FLS, which might provide novel targets for the treatment of RA.\n                  <\/jats:p>","DOI":"10.1155\/2020\/9510594","type":"journal-article","created":{"date-parts":[[2020,10,22]],"date-time":"2020-10-22T15:35:08Z","timestamp":1603380908000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Knockdown of YAP\/TAZ Inhibits the Migration and Invasion of Fibroblast Synovial Cells in Rheumatoid Arthritis by Regulating 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