{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T16:34:22Z","timestamp":1740155662946,"version":"3.37.3"},"reference-count":24,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T00:00:00Z","timestamp":1586304000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007939","name":"Rangsit University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007939","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology Research"],"published-print":{"date-parts":[[2020,4,8]]},"abstract":"<jats:p>Genetic diversity of <jats:italic>Plasmodium<\/jats:italic> parasite has significantly related to malaria control and vaccine development. The <jats:italic>P. falciparum<\/jats:italic> merozoite surface protein 1 (<jats:italic>Pfmsp1<\/jats:italic>) gene is a commonly used molecular marker to differentiate genetic diversity. This study is aimed at developing a nested PCR-Heteroduplex Mobility Assay (nPCR-HMA) for determination of the block 2 of the <jats:italic>Pfmsp1<\/jats:italic> gene. The MAD20 family allele of <jats:italic>P. falciparum<\/jats:italic> was used as a control for optimization of the annealing and polyacrylamide gel electrophoresis conditions. In order to evaluate the developed nPCR-HMA, 8 clinical <jats:italic>P. falciparum<\/jats:italic> isolates were examined for allelic variants. The results revealed 9 allelic variants. Our study indicated that the successful nPCR-HMA with good precision and accuracy offers a more rapid, efficient, and cheap method for large-scale molecular epidemiological studies as compared to nucleotide sequencing.<\/jats:p>","DOI":"10.1155\/2020\/9520326","type":"journal-article","created":{"date-parts":[[2020,4,8]],"date-time":"2020-04-08T23:32:31Z","timestamp":1586388751000},"page":"1-9","source":"Crossref","is-referenced-by-count":0,"title":["Development of Nested PCR-Heteroduplex Mobility Assay for Determination of Genetic Diversity in the Block 2 Region of the <i>Plasmodium falciparum<\/i> Merozoite Surface Protein 1 Gene"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7553-829X","authenticated-orcid":true,"given":"Kanyanan","family":"Kritsiriwuthinan","sequence":"first","affiliation":[{"name":"Faculty of Medical Technology, Rangsit University, Pathumthani, 12000, 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