{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T08:30:34Z","timestamp":1792225834669,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,8,17]],"date-time":"2020-08-17T00:00:00Z","timestamp":1597622400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["RSP-2020\/165"],"award-info":[{"award-number":["RSP-2020\/165"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","award":["BT\/PR12987\/INF\/22\/205\/2015"],"award-info":[{"award-number":["BT\/PR12987\/INF\/22\/205\/2015"]}],"id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Department of Biotechnology, Govt. of India","award":["RSP-2020\/165"],"award-info":[{"award-number":["RSP-2020\/165"]}]},{"name":"Department of Biotechnology, Govt. of India","award":["BT\/PR12987\/INF\/22\/205\/2015"],"award-info":[{"award-number":["BT\/PR12987\/INF\/22\/205\/2015"]}]},{"name":"SRM Institute of Science and Technology","award":["RSP-2020\/165"],"award-info":[{"award-number":["RSP-2020\/165"]}]},{"name":"SRM Institute of Science and Technology","award":["BT\/PR12987\/INF\/22\/205\/2015"],"award-info":[{"award-number":["BT\/PR12987\/INF\/22\/205\/2015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2020,8,17]]},"abstract":"<jats:p>The genetic polymorphism in the nuclear factor erythroid 2-related factor 2 (Nrf2) gene has been reported as one of the prognosis markers for various diseases, including cancer. Nrf2 is a key transcription factor involved in wound healing by regulating angiogenesis. We investigated the genetic association of <jats:italic>NRF2<\/jats:italic> single-nucleotide polymorphism rs35652124 with T2DM and DFU and assessed its functional impact. A total of 400 subjects were recruited for the study and categorized into three groups: infected DFU patients (DFU, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>100<\/mml:mn><\/mml:math>), T2DM patients without complications (T2DM, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>150<\/mml:mn><\/mml:math>), and healthy adults with normal glucose tolerance (NGT, <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>150<\/mml:mn><\/mml:math>). The subjects were genotyped by PCR-RFLP, and the polymorphism was identified by bidirectional Sanger sequencing. The expression of <jats:italic>NRF2<\/jats:italic>, <jats:italic>IL-10<\/jats:italic>, <jats:italic>TNF-\u03b1<\/jats:italic>, and <jats:italic>IL-6<\/jats:italic> was studied by qPCR to evaluate the functional impact of rs35652124. The \u201cTT\u201d genotype of rs35652124 was associated with a significant risk for T2DM [<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mtext>OR<\/mml:mtext><mml:mo>=<\/mml:mo><mml:mn>2.2<\/mml:mn><\/mml:math> (1.2-4.2), <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>p<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>] and DFU [<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mtext>OR<\/mml:mtext><mml:mo>=<\/mml:mo><mml:mn>7.9<\/mml:mn><\/mml:math> (4-14.9), <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.0001<\/mml:mn><\/mml:math>]. A significant decrease in transcriptional levels of <jats:italic>NRF2<\/jats:italic> and <jats:italic>IL-10<\/jats:italic> and a remarkable increase in <jats:italic>TNF-\u03b1<\/jats:italic> and <jats:italic>IL-6<\/jats:italic> were observed in subjects with TT genotype. In conclusion, rs35652124 (TT) is a harmful genetic variant that predisposes to insulin resistance and impaired angiogenesis. Hence, it may serve as a diagnostic genetic marker for T2DM and DFU in combination with different inflammatory markers.<\/jats:p>","DOI":"10.1155\/2020\/9825028","type":"journal-article","created":{"date-parts":[[2020,8,18]],"date-time":"2020-08-18T00:16:02Z","timestamp":1597709762000},"page":"1-9","source":"Crossref","is-referenced-by-count":25,"title":["Genetic Polymorphism of the Nrf2 Promoter Region (rs35652124) Is Associated with the Risk of Diabetic Foot Ulcers"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9989-909X","authenticated-orcid":true,"given":"Rajan","family":"Teena","sequence":"first","affiliation":[{"name":"Department of Biotechnology and SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Umapathy","family":"Dhamodharan","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daoud","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kesavan","family":"Rajesh","sequence":"additional","affiliation":[{"name":"Department of Podiatry, Hycare Super Speciality Hospital, MMDA Colony, Arumbakkam, Chennai, Tamil Nadu, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5450-902X","authenticated-orcid":true,"given":"Kunka Mohanram","family":"Ramkumar","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, 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