{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T05:04:21Z","timestamp":1793163861969,"version":"4.5.0"},"reference-count":71,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,20]],"date-time":"2021-11-20T00:00:00Z","timestamp":1637366400000},"content-version":"vor","delay-in-days":323,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31771491"],"award-info":[{"award-number":["31771491"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2018YFA0107900"],"award-info":[{"award-number":["2018YFA0107900"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    <jats:italic>Background<\/jats:italic>\n                    . Mitochondrial dysfunctions play a pivotal role in cerebral ischemia\u2010reperfusion (I\/R) injury. Although mitochondrial transplantation has been recently explored for the treatment of cerebral I\/R injury, the underlying mechanisms and fate of transplanted mitochondria are still poorly understood.\n                    <jats:italic>Methods<\/jats:italic>\n                    . Mitochondrial morphology and function were assessed by fluorescent staining, electron microscopy, JC\u20101, PCR, mitochondrial stress testing, and metabolomics. Therapeutic effects of mitochondria were evaluated by cell viability, reactive oxygen species (ROS), and apoptosis levels in a cellular hypoxia\u2010reoxygenation model. Rat middle cerebral artery occlusion model was applied to assess the mitochondrial therapy in vivo. Transcriptomics was performed to explore the underlying mechanisms. Mitochondrial fate tracking was implemented by a variety of fluorescent labeling methods.\n                    <jats:italic>Results<\/jats:italic>\n                    . Neuro\u20102a (N2a) cell\u2010derived mitochondria had higher mitochondrial membrane potential, more active oxidative respiration capacity, and less mitochondrial DNA copy number. Exogenous mitochondrial transplantation increased cellular viability in an oxygen\u2010dependent manner, decreased ROS and apoptosis levels, improved neurobehavioral deficits, and reduced infarct size. Transcriptomic data showed that the differential gene enrichment pathways are associated with metabolism, especially lipid metabolism. Mitochondrial tracking indicated specific parts of the exogenous mitochondria fused with the mitochondria of the host cell, and others were incorporated into lysosomes. This process occurred at the beginning of internalization and its efficiency is related to intercellular connection.\n                    <jats:italic>Conclusions<\/jats:italic>\n                    . Mitochondrial transplantation may attenuate cerebral I\/R injury. The mechanism may be related to mitochondrial component separation, altering cellular metabolism, reducing ROS, and apoptosis in an oxygen\u2010dependent manner. The way of isolated mitochondrial transfer into the cell may be related to intercellular connection.\n                  <\/jats:p>","DOI":"10.1155\/2021\/1006636","type":"journal-article","created":{"date-parts":[[2021,11,20]],"date-time":"2021-11-20T13:35:06Z","timestamp":1637415306000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Mitochondrial Transplantation Attenuates Cerebral Ischemia\u2010Reperfusion Injury: Possible Involvement of Mitochondrial Component 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