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However, there is limited data on the role of HDV in the oncogenesis of HCC. This study is aimed at assessing the potential mechanisms of HDV\u2010associated hepatocarcinogenesis, especially to screen and identify key genes and pathways possibly involved in the pathogenesis of HCC. We selected three microarray datasets: GSE55092 contains 39 cancer specimens and 81 paracancer specimens from 11 HBV\u2010associated HCC patients, GSE98383 contains 11 cancer specimens and 24 paracancer specimens from 5 HDV\u2010associated HCC patients, and 371 HCC patients with the RNA\u2010sequencing data combined with their clinical data from the Cancer Genome Atlas (TCGA). Afterwards, 948 differentially expressed genes (DEGs) closely related to HDV\u2010associated HCC were obtained using the R package and filtering with a Venn diagram. We then performed gene ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis to determine the biological processes (BP), cellular component (CC), molecular function (MF), and KEGG signaling pathways most enriched for DEGs. Additionally, we performed Weighted Gene Coexpression Network Analysis (WGCNA) and protein\u2010to\u2010protein interaction (PPI) network construction with 948 DEGs, from which one module was identified by WGCNA and three modules were identified by the PPI network. Subsequently, we validated the expression of 52 hub genes from the PPI network with an independent set of HCC dataset stored in the Gene Expression Profiling Interactive Analysis (GEPIA) database. Finally, seven potential key genes were identified by intersecting with key modules from WGCNA, including 3 reported genes, namely, <jats:italic>CDCA5<\/jats:italic>, <jats:italic>CENPH<\/jats:italic>, and <jats:italic>MCM7<\/jats:italic>, and 4 novel genes, namely, <jats:italic>CDC6<\/jats:italic>, <jats:italic>CDC45<\/jats:italic>, <jats:italic>CDCA8<\/jats:italic>, and <jats:italic>MCM4<\/jats:italic>, which are associated with nucleoplasm, cell cycle, DNA replication, and mitotic cell cycle. The <jats:italic>CDCA8<\/jats:italic> and stage of HCC were the independent factors associated with overall survival of HDV\u2010associated HCC. All the related findings of these genes can help gain a better understanding of the role of HDV in the underlying mechanism of HCC carcinogenesis.<\/jats:p>","DOI":"10.1155\/2021\/1093702","type":"journal-article","created":{"date-parts":[[2021,1,31]],"date-time":"2021-01-31T03:20:10Z","timestamp":1612063210000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Microarray Data Mining and Preliminary Bioinformatics Analysis of Hepatitis D Virus\u2010Associated Hepatocellular Carcinoma"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4115-3332","authenticated-orcid":false,"given":"Zhe","family":"Yu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1122-722X","authenticated-orcid":false,"given":"Xuemei","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4961-8560","authenticated-orcid":false,"given":"Xiujuan","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4629-6770","authenticated-orcid":false,"given":"Linjing","family":"An","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9101-726X","authenticated-orcid":false,"given":"Ming","family":"Niu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6706-6301","authenticated-orcid":false,"given":"Yan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2910-0481","authenticated-orcid":false,"given":"Chao","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8307-1095","authenticated-orcid":false,"given":"Yongping","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,30]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21492"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1713263"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21338"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268818001942"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31865-8"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.3795"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-46493-1"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/apt.15188"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-26446-w"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.02.013"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-18-0012"},{"key":"e_1_2_10_12_2","volume-title":"Hepatitis viruses","author":"Humans, I.W.G.o.t.E.o.C.R.t","year":"1994"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-7645-0_7"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn764"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.207"},{"key":"e_1_2_10_16_2","first-page":"397","volume-title":"Bioinformatics and computational biology solutions using R and Bioconductor","author":"Smyth G. 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