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Genes encoding antimicrobial resistance are mainly harbored on mobile genetic elements (MGEs) such as transposons and plasmids as well as integrons, which enhance their rapid spread. The aim of this study was to characterize 83 multiantimicrobial\u2010resistant <jats:italic>E. coli<\/jats:italic> isolates recovered from healthy broiler chickens. Among 78 tetracycline\u2010resistant isolates, the <jats:italic>tetA<\/jats:italic>, <jats:italic>tetB<\/jats:italic>, and <jats:italic>tetC<\/jats:italic> genes were detected in 59 (75.6%), 14 (17.9%), and one (1.2%) isolates, respectively. The <jats:italic>sul1<\/jats:italic>, <jats:italic>sul2<\/jats:italic>, and <jats:italic>sul3<\/jats:italic> genes were detected 31 (46.2%), 16 (23.8%), and 6 (8.9%) isolates, respectively, among 67 sulfonamide\u2010resistant isolates. The PCR\u2010based replicon typing method showed plasmids in 29 isolates, IncFIB (19), IncI1\u2010I<jats:italic>\u03b3<\/jats:italic> (17), IncF (14), IncK (14), IncFIC (10), IncP (8), IncY (3), IncHI2 (1), and IncX (1). The class 1 and 2 integrons were detected in 57 and 2 isolates, respectively; one isolate harbored both integrons. Seven and one gene cassette arrays were identified in class 1 and class 2 integrons, respectively. Our findings show that multiantimicrobial\u2010resistant <jats:italic>E. coli<\/jats:italic> isolates from chickens serve as reservoirs of highly diverse and abundant <jats:italic>tet<\/jats:italic> and <jats:italic>sul<\/jats:italic> genes and plasmid replicons. Such isolates and MGEs pose a potential health threat to the public and animal farming.<\/jats:p>","DOI":"10.1155\/2021\/1269849","type":"journal-article","created":{"date-parts":[[2021,10,2]],"date-time":"2021-10-02T01:36:10Z","timestamp":1633138570000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Genetic Background of Antimicrobial Resistance in Multiantimicrobial\u2010Resistant <i>Escherichia coli<\/i> Isolates from Feces of Healthy Broiler Chickens in Tunisia"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9673-193X","authenticated-orcid":false,"given":"Mohamed 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