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To this aim, we used sixteen fresh sperm samples from patients undergoing ART for male infertility and classified them in the high\u2010 and poor\u2010quality groups, on the basis of their morphology at high magnification. Then, using a DNA sequencing method, we analyzed the spermatozoa methylome to identify genes that were differentially methylated. By Gene Ontology and protein\u2013protein interaction network analyses, we defined candidate genes mainly implicated in cell motility, calcium reabsorption, and signaling pathways as well as transmembrane transport. RT\u2010qPCR of high\u2010 and poor\u2010quality sperm samples allowed showing that the expression of some genes, such as <jats:italic>AURKA<\/jats:italic>, <jats:italic>HDAC4<\/jats:italic>, <jats:italic>CFAP46<\/jats:italic>, <jats:italic>SPATA18<\/jats:italic>, <jats:italic>CACNA1C<\/jats:italic>, <jats:italic>CACNA1H<\/jats:italic>, <jats:italic>CARHSP1<\/jats:italic>, <jats:italic>CCDC60<\/jats:italic>, <jats:italic>DNAH2<\/jats:italic>, and <jats:italic>CDC88B<\/jats:italic>, have different expression levels according to sperm morphology. In conclusion, the present study shows a strong correlation between morphology and gene expression in the spermatozoa and provides a biomarker panel for sperm analysis during ART and a new tool to explore male infertility.<\/jats:p>","DOI":"10.1155\/2021\/1434546","type":"journal-article","created":{"date-parts":[[2021,9,21]],"date-time":"2021-09-21T04:05:07Z","timestamp":1632197107000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Molecular Profiling of Spermatozoa Reveals Correlations between Morphology and Gene Expression: A Novel Biomarker Panel for Male Infertility"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4586-4369","authenticated-orcid":false,"given":"Nino 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