{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T21:22:57Z","timestamp":1783286577284,"version":"3.54.6"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T00:00:00Z","timestamp":1632096000000},"content-version":"vor","delay-in-days":262,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004919","name":"King Abdulaziz City for Science and Technology","doi-asserted-by":"publisher","award":["14-MED333-10"],"award-info":[{"award-number":["14-MED333-10"]}],"id":[{"id":"10.13039\/501100004919","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>The aim of the present investigation was to evaluate the effect of the combined crude extract, fractions, and compound 1 isolated from the fruits of <jats:italic>Datura metel<\/jats:italic> against selected microbial (bacteria and fungi) strains. Results of antibacterial screening indicated marked susceptibility of the extract and its fractions against tested bacterial strains. Among the extract and various fractions, the chloroform fraction exhibited a significant effect against different bacterial strains including <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Staphylococcus aureus<\/jats:italic>, and <jats:italic>Bacillus subtilis<\/jats:italic> with an inhibitory zone ranging from 18 to 24\u2009mm. Similarly, results of antifungal activity revealed that the chloroform fraction displays a promising effect against various fungal strains. The chloroform fraction was subjected to repeated chromatography analysis, which yielded compound <jats:bold>1<\/jats:bold> (daturaolone). Daturaolone exhibited potent activity against selected bacterial strains including <jats:italic>Klebsiella pneumoniae<\/jats:italic>, <jats:italic>B. subtilis<\/jats:italic>, <jats:italic>S. epidermidis<\/jats:italic>, and <jats:italic>S. aureus<\/jats:italic> with an inhibitory zone ranging from 12 to 30\u2009mm. In addition, the extracts and daturaolone exhibited significant sensitivity against <jats:italic>T. longifusus<\/jats:italic>, <jats:italic>C. albicans<\/jats:italic>, <jats:italic>A. flavus<\/jats:italic>, <jats:italic>M. canis<\/jats:italic>, <jats:italic>F. solani<\/jats:italic>, and <jats:italic>C. glabrata<\/jats:italic>. Taken all together, it is concluded that our findings validated the traditional use of <jats:italic>D. metel<\/jats:italic> to treat various infectious diseases, which is supported by daturaolone.<\/jats:p>","DOI":"10.1155\/2021\/1543574","type":"journal-article","created":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T21:09:49Z","timestamp":1632172189000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["In Vitro Antibacterial and Antifungal Potential of Amyrin\u2010Type Triterpenoid Isolated from <i>Datura metel<\/i> Linnaeus"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3140-5153","authenticated-orcid":false,"given":"Sami","family":"Bawazeer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2429-5491","authenticated-orcid":false,"given":"Abdur","family":"Rauf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,20]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.1c00537"},{"key":"e_1_2_9_2_2","first-page":"123","article-title":"Review on Datura metel: a potential medicinal plant","volume":"1","author":"Khaton M. 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