{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T18:43:37Z","timestamp":1761677017266,"version":"3.41.2"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"vor","delay-in-days":264,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31902289"],"award-info":[{"award-number":["31902289"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> is a protozoan parasite that is widely parasitic in the nucleated cells of warm\u2010blooded animals. Bioinformatic analysis of alkyl hydroperoxide reductase 1 (AHP1) of <jats:italic>T. gondii<\/jats:italic> is a member of the Prxs family and exhibits peroxidase activity. Cys<jats:sup>166<\/jats:sup> was certified to be a key enzyme active site of TgAHP1, indicating that the enzyme follows a cysteine\u2010dependent redox process. TgAHP1 was present in a punctate staining pattern anterior to the <jats:italic>T. gondii<\/jats:italic> nucleus. Oxidative stress experiments showed that the \u2206Ahp1 strain was more sensitive to tert\u2010butyl hydroperoxide (tBOOH) than hydrogen peroxide (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>), indicating that tBOOH may be a sensitive substrate for TgAHP1. Under tBOOH culture conditions, the \u2206Ahp1 strain was significantly less invasive, proliferative, and pathogenic in mice. This was mainly due to the induction of tBOOH, which increased the level of reactive oxygen species in the parasites and eventually led to apoptosis. This study shows that TgAHP1 is a peroxisomes protein with cysteine\u2010dependent peroxidase activity and sensitive to tBOOH.<\/jats:p>","DOI":"10.1155\/2021\/1675652","type":"journal-article","created":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T16:05:45Z","timestamp":1632326745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Alkyl Hydroperoxide Reductase as a Determinant of Parasite Antiperoxide Response in <i>Toxoplasma 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