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Propionibacterium acnes (P. acnes) hypercolonization is one pathogenic factor for AV. P. acnes that triggers interleukin-1\u03b2 (IL-1\u03b2) by activating the pyrin domain-containing 3 protein (NLRP3) inflammasome of the NOD-like receptor family in human monocytes. Reactive oxygen species (ROS) acts as a trigger for the production of IL-8 and activates theNLRP3 inflammasome. IL-8 promotes the metastasis and multiplication of different cancerous cells, whereas keratinocyte proliferation and migration contribute to the progression of AV. A steroidal saponin called polyphyllin I (PPI) that is extracted from Paris polyphylla\u2019s rhizomes has anti-inflammatory properties. This study investigates the regulatory role of P. acnes in the secretion of IL-8 mediated by the CD36\/NADPH oxidase 1 (NOX1)\/ROS\/NLRP3\/IL-1\u03b2 pathway and the effects of PPI on the CD36\/NOX1\/ROS\/NLRP3\/IL-1\u03b2\/IL-8 pathway and human keratinocyte proliferation and migration. HaCaT cells were cultured and stimulated with 108\u2009CFU\/ml of P. acnes for 0, 6, 12, 18, 24, 30, and 36 hours. P. acnes induced IL-8 secretion from HaCaT cells via the CD36\/NOX1\/ROS\/NLRP3\/IL-1\u03b2 pathway. PPI inhibited the CD36\/NLRP3\/NOX1\/ROS\/IL-8\/IL-1\u03b2 pathway and HaCaT cell proliferation and migration. PPI alleviates P. acnes-induced inflammatory responses and human keratinocyte proliferation and migration, implying a novel potential therapy for AV.<\/jats:p>","DOI":"10.1155\/2021\/1821220","type":"journal-article","created":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T18:26:50Z","timestamp":1632335210000},"page":"1-11","source":"Crossref","is-referenced-by-count":14,"title":["Polyphyllin I Inhibits Propionibacterium acnes-Induced IL-8 Secretion in HaCaT Cells by Downregulating the CD36\/NOX1\/ROS\/NLRP3\/IL-1\u03b2 Pathway"],"prefix":"10.1155","volume":"2021","author":[{"given":"Shuyun","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Dermatology, Baoshan People\u2019s Hospital, Baoshan, Yunnan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 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