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A total of 240 1\u2010day\u2010old Arbor Acres chicks were divided into four groups, as negative controls (NC), positive controls (PC), berberine\u2010 (BER\u2010) treated, or lincomycin\u2010 (LMY\u2010) treated groups. Broilers were challenged with <jats:italic>C. perfringens<\/jats:italic> at 15\u201021 days of age, followed by BER or LMY supplied in drinking water for 7 days. Experimental results showed that <jats:italic>C. perfringens<\/jats:italic> infection significantly decreased growth performance and increased intestinal necrosis index and the number of <jats:italic>C. perfringens<\/jats:italic> present to 6.45 Log<jats:sub>10<\/jats:sub>CFU\/g (<jats:italic>P<\/jats:italic> &lt; 0.001). Proinflammatory cytokines in the ileum were significantly increased, but the expression of ileal tight junction proteins occludin and claudin\u20101 was significantly reduced. Both BER and LMY ameliorated some of these observations. Compared with the PC group, the number of <jats:italic>C. perfringens<\/jats:italic> in the cecum was significantly decreased following treatment (<jats:italic>P<\/jats:italic> &lt; 0.001), and growth performance and small intestine morphology were similar to those of the NC group (<jats:italic>P<\/jats:italic> &gt; 0.05). IL\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20106, and TNF\u2010<jats:italic>\u03b1<\/jats:italic> levels as well as occludin and claudin\u20101 expression were also significantly improved (<jats:italic>P<\/jats:italic> &lt; 0.05). BER has the potential to replace antibiotics for NE caused by <jats:italic>C. perfringens<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2021\/1877075","type":"journal-article","created":{"date-parts":[[2021,10,20]],"date-time":"2021-10-20T15:04:39Z","timestamp":1634742279000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Impact of Berberine on Intestinal Morphology, Microbes, and Immune Function of Broilers in Response to Necrotic Enteritis 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