{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T03:24:05Z","timestamp":1772767445392,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,8,10]],"date-time":"2021-08-10T00:00:00Z","timestamp":1628553600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pearl River Talents Scheme of Guangdong Province","award":["00201512"],"award-info":[{"award-number":["00201512"]}]},{"name":"Pearl River Talents Scheme of Guangdong Province","award":["81871227"],"award-info":[{"award-number":["81871227"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["00201512"],"award-info":[{"award-number":["00201512"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871227"],"award-info":[{"award-number":["81871227"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2021,8,10]]},"abstract":"<jats:p>The presence of anti-citrullinated protein antibodies (ACPAs) in the serum is one of the immunological features of rheumatoid arthritis (RA). Anti-cyclic citrullinated peptide (CCP) assay has been widely used in clinic for the diagnosis of RA. However, up to 40% of RA patients are anti-CCP negative and the diagnostic sensitivity in this population needs to be improved for better clinical management. In this study, peptides with Multiple Citrulline Similar Motif (MCSM) were synthesized and a new ELISA system, which we called RA_CP, was developed to detect citrullinated antigens with MCSM present in the serum. 106 RA,48 other arthritis patients and 41 sex- and age-matched healthy controls (HCs) were included in this study. Patients with RA have a significantly higher amount of citrullinated antigens with MCSM than other arthritis patients and HCs. RA patients with positive anti-CCP are also MCSM positive, whereas 75% anti-CCP negative patients are positive for MCSM. The diagnostic sensitivity for anti-CCP and MCSM was 81.1% and 95.3%, while the specificity was 100% and 94.4%, respectively. ROC curve analyses showed that the area under the curve (AUC) values were 0.906 (95% CI: 0.860-0.951) for anti-CCP and 0.948 (95% CI: 0.912-0.985) for MCSM while the combination of MCSM and anti-CCP test has the highest AUC (0.971, 95% CI: 0.946\u20130.996). Our results suggest that detection of citrullinated antigens with MCSM has improved sensitivity compared with anti-CCP assay and could serve as a biomarker in diagnosis of RA patients.<\/jats:p>","DOI":"10.1155\/2021\/1891519","type":"journal-article","created":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T23:05:10Z","timestamp":1628809510000},"page":"1-6","source":"Crossref","is-referenced-by-count":5,"title":["Citrullinated Antigens with Multiple Citrulline Similar Motif in the Diagnosis of Rheumatoid Arthritis: A Preliminary Single-Center Study"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0828-1583","authenticated-orcid":true,"given":"Zhu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Rheumatology and Immunology, Anhui Medical University Affiliated Provincial Hospital, Lujiang Str 17, Hefei 230001, China"},{"name":"Department of Rheumatology and Immunology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiting","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, Anhui Medical University Affiliated Provincial Hospital, Lujiang Str 17, Hefei 230001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7515-5918","authenticated-orcid":true,"given":"Yi","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin-Zhao","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Immunology, Anhui Medical University Affiliated Provincial Hospital, Lujiang Str 17, Hefei 230001, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Leide Biosciences Co., Ltd., Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianrong","family":"Lou","sequence":"additional","affiliation":[{"name":"Leide Biosciences Co., Ltd., Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2018.1"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.138461"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1111\/1756-185X.13459"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-013-0366-7"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1136\/rmdopen-2019-000969"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-019-04492-5"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1186\/s13075-019-1909-8"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1316"},{"issue":"1","key":"9","first-page":"144","article-title":"The performance of anti-cyclic citrullinated peptide assays in diagnosing rheumatoid arthritis: a systematic review and meta-analysis","volume":"36","author":"L. Mathsson Alm","year":"2018","journal-title":"Clinical and Experimental Rheumatology"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1186\/ar2903"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2018.02.014"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12834"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21114015"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-008-0999-5"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2012.09.015"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2018.03.002"},{"issue":"4","key":"17","first-page":"612","article-title":"Serum IgG ACPA-IgM RF immune complexes were detected in rheumatoid arthritis patients positive for IgM ACPA","volume":"36","author":"A. Haraguchi","year":"2018","journal-title":"Clinical and Experimental Rheumatology"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1002\/art.40578"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2021\/1891519.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2021\/1891519.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2021\/1891519.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,8,12]],"date-time":"2021-08-12T23:05:15Z","timestamp":1628809515000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jir\/2021\/1891519\/"}},"subtitle":[],"editor":[{"given":"Yan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,8,10]]},"references-count":18,"alternative-id":["1891519","1891519"],"URL":"https:\/\/doi.org\/10.1155\/2021\/1891519","relation":{},"ISSN":["2314-7156","2314-8861"],"issn-type":[{"value":"2314-7156","type":"electronic"},{"value":"2314-8861","type":"print"}],"subject":[],"published":{"date-parts":[[2021,8,10]]}}}