{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T13:18:41Z","timestamp":1753881521472,"version":"3.41.2"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,1,27]],"date-time":"2021-01-27T00:00:00Z","timestamp":1611705600000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Acellular allograft (ACA) improves the repair and reconstruction of long peripheral nerve defects. <jats:italic>\u03c9<\/jats:italic>\u20103 Polyunsaturated fatty acids (PUFAs) carry a neuroprotective potential, and their effects on ACA bridging were elucidated. Thirty rats with long gap sciatic nerve defects (15\u2009mm long) were randomly divided into three groups (<jats:italic>n<\/jats:italic> = 10): ACA, ACA\u2009+\u2009PUFAs, and autograft (AU). Limb condition, wet weight of tibialis anterior muscle (TAM), nerve electrophysiology, S\u2010100, horseradish peroxidase (HRP), and percentage of splenic CD4+ and CD8\u2009+\u2009T\u2010lymphocytes were evaluated for 12 weeks after the operation. Rats in the AU and ACA\u2009+\u2009PUFA groups showed superior condition in affected limbs compared to the ACA group. At 12\u2009wk after surgery, the wet weight of TAM in the ACA\u2009+\u2009PUFA group was higher than that in the ACA group (0.4519 \u00b1 0.1185 vs. 0.3049 \u00b1 0.1272; <jats:italic>P<\/jats:italic> &lt; 0.01) but lower than that in the AU group (0.4519 \u00b1 0.1185, 0.5628 \u00b1 0.0092; <jats:italic>P<\/jats:italic> &lt; 0.05). In all the three groups, sole irritation elicited withdrawal reflex, and S\u2010100 staining was detected in plantar skin. Moreover, horseradish peroxidase staining was overt in both the ventral horn and dorsal root ganglion of the spinal cord. Nerve conduction velocity (m\/s), amplitude of action potential (mV), or somatosensory evoked potentials in ACA\u2009+\u2009PUFAs (28.81 \u00b1 1.04, 2.20 \u00b1 0.27, 6.98 \u00b1 0.29) were significantly different from that in the AU (35.71 \u00b1 1.28, 1.81 \u00b1 0.19, 8.15 \u00b1 0.52; <jats:italic>P<\/jats:italic> &lt; 0.05) and ACA (20.03 \u00b1 1.94, 2.95 \u00b1 0.36, 5.22 \u00b1 0.53; <jats:italic>P<\/jats:italic> &lt; 0.01) groups. The percentages of splenic CD4+ and CD8+ cells were similar among the three groups. Omega\u20103 PUFAs improve the bridging effect of ACA on long gap peripheral nerve defects by promoting neuroprotection without arousing an immune response.<\/jats:p>","DOI":"10.1155\/2021\/2504276","type":"journal-article","created":{"date-parts":[[2021,1,28]],"date-time":"2021-01-28T03:20:08Z","timestamp":1611804008000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>\u03c9<\/i>\u20103 Polyunsaturated Fatty Acids Facilitate the Repair of Peripheral Nerve Defects with Chemically Extracted Acellular Allograft in Rats"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8962-616X","authenticated-orcid":false,"given":"Jun","family":"Zuo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renkun","family":"Xiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiping","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2617-1847","authenticated-orcid":false,"given":"Yi","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,27]]},"reference":[{"key":"e_1_2_8_1_2","first-page":"100","article-title":"Neurobiology of peripheral nerve injury, regeneration, and functional recovery: from bench top research to bedside application","volume":"13","author":"Sulaiman W.","year":"2013","journal-title":"Ochsner Journal"},{"doi-asserted-by":"publisher","key":"e_1_2_8_2_2","DOI":"10.1055\/s-2003-40580"},{"doi-asserted-by":"publisher","key":"e_1_2_8_3_2","DOI":"10.1016\/j.neulet.2012.02.066"},{"doi-asserted-by":"publisher","key":"e_1_2_8_4_2","DOI":"10.1016\/j.expneurol.2010.01.022"},{"doi-asserted-by":"publisher","key":"e_1_2_8_5_2","DOI":"10.1097\/NEN.0b013e3181eff6dc"},{"doi-asserted-by":"publisher","key":"e_1_2_8_6_2","DOI":"10.1016\/S0749-0712(21)00506-0"},{"doi-asserted-by":"publisher","key":"e_1_2_8_7_2","DOI":"10.1016\/S0006-8993(98)00251-0"},{"doi-asserted-by":"publisher","key":"e_1_2_8_8_2","DOI":"10.1111\/j.1753-4887.2010.00287.x"},{"doi-asserted-by":"publisher","key":"e_1_2_8_9_2","DOI":"10.2741\/3878"},{"doi-asserted-by":"publisher","key":"e_1_2_8_10_2","DOI":"10.14336\/AD.2017.0520"},{"doi-asserted-by":"publisher","key":"e_1_2_8_11_2","DOI":"10.1074\/jbc.M305841200"},{"doi-asserted-by":"publisher","key":"e_1_2_8_12_2","DOI":"10.1172\/JCI25420"},{"doi-asserted-by":"publisher","key":"e_1_2_8_13_2","DOI":"10.1016\/0304-3959(83)90201-4"},{"key":"e_1_2_8_14_2","article-title":"Promotion effects of nerve regeneration of chemically extracted acellular nerve allograft in vitro","author":"Wang Y.","year":"2010","journal-title":"Orthopedic Journal of China"},{"doi-asserted-by":"publisher","key":"e_1_2_8_15_2","DOI":"10.1002\/(sici)1097-4598(200006)23:6<863::aid-mus4>3.0.co;2-0"},{"doi-asserted-by":"publisher","key":"e_1_2_8_16_2","DOI":"10.1007\/s10354-018-0675-6"},{"doi-asserted-by":"publisher","key":"e_1_2_8_17_2","DOI":"10.1097\/BCO.0b013e31829fc54b"},{"doi-asserted-by":"publisher","key":"e_1_2_8_18_2","DOI":"10.1016\/j.neuroscience.2019.06.027"},{"doi-asserted-by":"publisher","key":"e_1_2_8_19_2","DOI":"10.1016\/j.nbd.2016.02.020"},{"doi-asserted-by":"publisher","key":"e_1_2_8_20_2","DOI":"10.1016\/0090-1229(92)90078-3"},{"doi-asserted-by":"publisher","key":"e_1_2_8_21_2","DOI":"10.1179\/107902611x12972448729521"},{"key":"e_1_2_8_22_2","article-title":"Serum and cerebrospinal fluid levels of S-100\u03b2 is a biomarker for spinal cord injury; 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