{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T02:46:59Z","timestamp":1769050019156,"version":"3.49.0"},"reference-count":64,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T00:00:00Z","timestamp":1611964800000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Research Projects of Health and Family Planning Commission of Chongqing","award":["2017MSXM017"],"award-info":[{"award-number":["2017MSXM017"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Background.<\/jats:italic> Liver macrophages play an important regulatory role in the inflammatory response of liver injury after severe infection. Interleukin\u2010 (IL\u2010) 27 is an inflammatory cytokine that plays an important role in diseases caused by bacterial infection. However, the relationship between IL\u201027 and liver macrophages in liver injury after severe infection is not yet clear. <jats:italic>Methods.<\/jats:italic> A cecal ligation puncture (CLP) model was established in wild\u2010type (WT) and IL\u201027 receptor\u2010 (WSX\u20101\u2010) deficient (IL\u201027r<jats:sup>-\/-<\/jats:sup>) mice, and recombinant IL\u201027 and gadolinium chloride (GdCl3) were injected into WT mice in the designated groups. The serum and liver IL\u201027, IL\u20106, tumor necrosis factor alpha (TNF\u2010<jats:italic>\u03b1<\/jats:italic>), and IL\u20101<jats:italic>\u03b2<\/jats:italic> expression levels were evaluated by ELISA, quantitative PCR, or Western blotting; serum ALT and AST were detected by detection kits; and the severity of liver damage was evaluated by hematoxylin and eosin staining and the TUNEL assay of the liver tissue from the different groups. Liver macrophage polarization was evaluated by immunofluorescence. In addition, the polarization of peritoneal macrophage was evaluated by flow cytometry. <jats:italic>Results.<\/jats:italic> The serum and liver IL\u201027 expression levels were elevated in WT mice after CLP\u2010induced severe infection, which were consistent with the changes in HE scores in the liver tissue. The levels of serum ALT, AST, liver IL\u20106, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, and IL\u20101<jats:italic>\u03b2<\/jats:italic> mRNA and liver pathological injury scores were further increased when pretreated with recombinant IL\u201027 in WT mice, but these levels were decreased in IL\u201027r<jats:sup>\u2212\/\u2212<\/jats:sup> mice after CLP\u2010induced severe infection compared to WT mice. In WT mice pretreated with GdCl3, liver pathological scores, serum ALT and AST, TUNEL\u2010positive cell proportion from liver tissues, liver IL\u201027 expression, and the liver macrophages M1 polarization proportion decreased after CLP; however, the serum IL\u201027, IL\u20106, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, and IL\u20101<jats:italic>\u03b2<\/jats:italic> levels and the pathological lung and kidney scores were not significantly changed. When supplemented with exogenous IL\u201027, the liver pathological scores, serum ALT, AST, TUNEL\u2010positive cell proportion of liver tissues, liver IL\u201027 expression, and the liver macrophage M1 polarization proportion increased. The in vitro, IL\u201027 expression increased in peritoneal macrophages when stimulated with LPS. Recombinant IL\u201027 together with LPS promoted the elevations in IL\u20106, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, and IL\u20101<jats:italic>\u03b2<\/jats:italic> levels in supernatant and the M1 polarization of peritoneal macrophages. <jats:italic>Conclusion.<\/jats:italic> IL\u201027 is an important cytokine in the inflammatory response to liver injury after severe infection. The reduction of liver injury by gadolinium chloride in severe infection mice models may relate to the inhibition of liver IL\u201027 production. These changes may be mainly related to the decrease of liver macrophages M1 polarization. IL\u201027 may have a positive feedback on these macrophages.<\/jats:p>","DOI":"10.1155\/2021\/2605973","type":"journal-article","created":{"date-parts":[[2021,1,30]],"date-time":"2021-01-30T13:20:10Z","timestamp":1612012810000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Gadolinium Chloride Inhibits the Production of Liver Interleukin\u201027 and Mitigates Liver Injury in the CLP Mouse Model"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4978-9455","authenticated-orcid":false,"given":"Jing","family":"Fan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miao","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuan-Jiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0763-9259","authenticated-orcid":false,"given":"Mu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,30]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCC.0b013e3282f6a435"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003246-200107000-00002"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1997.03550030074038"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/his.12364"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/08830185.2014.889129"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00324-2"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/thoraxjnl-2014-205777"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1541"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.106635"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20060471"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.1.30"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.11"},{"key":"e_1_2_10_13_2","article-title":"Phenotypical and functional polymorphism of liver resident macrophages","volume":"8","author":"Elchaninov A. V.","year":"2019","journal-title":"Cell"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0B013E31816532BE"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1208937"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394299-9.00001-1"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2003.08.004"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.11.015"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2012.48"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0000000000000755"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.214"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.3791\/55445"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002987"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2016.130"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V132431"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2019.154936"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc7157"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.09-2661"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000051515.56179.E1"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000284492.30800.00"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144278"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc10543"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001338"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e318266c6df"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2015.200"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-018-2180-0"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-015-1095-2"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/liv.12268"},{"key":"e_1_2_10_39_2","article-title":"Elevated serum IgG levels positively correlated with IL-27 may indicate poor outcome in patients with HBV-related acute-on-chronic liver failure","volume":"6","author":"Zhang G. L.","year":"2019","journal-title":"Journal of Immunology Research"},{"key":"e_1_2_10_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.08.015"},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc11381"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2011.08148.x"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510300104"},{"key":"e_1_2_10_44_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.2002.6434"},{"key":"e_1_2_10_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.03.009"},{"key":"e_1_2_10_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1527-3466.2001.tb00182.x"},{"key":"e_1_2_10_47_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.060010"},{"key":"e_1_2_10_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2006.02.067"},{"key":"e_1_2_10_49_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050599"},{"key":"e_1_2_10_50_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.00-0554fje"},{"key":"e_1_2_10_51_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00003.2003"},{"key":"e_1_2_10_52_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-9255-6-34"},{"key":"e_1_2_10_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24764"},{"key":"e_1_2_10_54_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2004.09.015"},{"key":"e_1_2_10_55_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-015-1995-y"},{"key":"e_1_2_10_56_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201900817R"},{"key":"e_1_2_10_57_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1402469"},{"key":"e_1_2_10_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2013.12.025"},{"key":"e_1_2_10_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.12.020"},{"key":"e_1_2_10_60_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10321"},{"key":"e_1_2_10_61_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20246207"},{"key":"e_1_2_10_62_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-17-0960"},{"key":"e_1_2_10_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2018.05.019"},{"key":"e_1_2_10_64_2","doi-asserted-by":"publisher","DOI":"10.1067\/msy.2003.189"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2021\/2605973.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/mi\/2021\/2605973.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2021\/2605973","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T09:31:30Z","timestamp":1756373490000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2021\/2605973"}},"subtitle":[],"editor":[{"given":"Michele T.","family":"Pritchard","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":64,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["10.1155\/2021\/2605973"],"URL":"https:\/\/doi.org\/10.1155\/2021\/2605973","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"2020-04-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2605973"}}