{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T18:02:36Z","timestamp":1788804156560,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,7,3]],"date-time":"2021-07-03T00:00:00Z","timestamp":1625270400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004413","name":"International Foundation for Science","doi-asserted-by":"publisher","award":["F\/5495-2"],"award-info":[{"award-number":["F\/5495-2"]}],"id":[{"id":"10.13039\/100004413","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004413","name":"International Foundation for Science","doi-asserted-by":"publisher","award":["3.4-8151"],"award-info":[{"award-number":["3.4-8151"]}],"id":[{"id":"10.13039\/100004413","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004413","name":"International Foundation for Science","doi-asserted-by":"publisher","award":["19061"],"award-info":[{"award-number":["19061"]}],"id":[{"id":"10.13039\/100004413","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","award":["F\/5495-2"],"award-info":[{"award-number":["F\/5495-2"]}],"id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","award":["3.4-8151"],"award-info":[{"award-number":["3.4-8151"]}],"id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","award":["19061"],"award-info":[{"award-number":["19061"]}],"id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,7,3]]},"abstract":"<jats:p>Arterial hypertension (AHT) is a leading cardiovascular disease, with a high negative impact on the quality of life. Crinum zeylanicum (C. zeylanicum) leaves extract is used in the West region of Cameroon to treat AHT and heart problems. This study aimed to investigate the antihypertensive effect of C. zeylanicum extract in N\u03c9-nitro-L\u2010arginine methyl ester- (L\u2010NAME-) induced hypertensive rats. The aqueous extract of C. zeylanicum (LAE) was obtained by lyophilizing the juice of triturated fresh leaves. The methanol extract (LME) prepared by maceration of the dried leaves was further partitioned to chloroform (LCF), ethyl acetate (LEAF), and residual (LRF) fractions. The total polyphenol, flavonoid content, and antiradical potentials of these extracts were determined. The curative antihypertensive and renal function protective effects of LME and LEAF were evaluated in vivo on L-NAME-induced hypertensive rats. Hypertension was induced in rats by oral administration of L-NAME (30\u2009mg\/kg\/day) for 3 consecutive weeks. Thereafter, plant extracts were administered orally at the doses of 30, 60, and 120\u2009mg\/kg\/day, concomitantly with L-NAME for three other weeks. Body weight, heart rate, and arterial blood pressure were measured at the end of each week throughout the experimental period. At the end of the treatment, 24-hour urine and plasma were collected to assay nitric oxide (NO), creatinine, and protein. The results revealed that LEAF has the higher content of total polyphenol and flavonoid and exhibited the best antiradical potential. Moreover, treatment of hypertensive rats with LME and LEAF significantly (<jats:inline-formula><a:math xmlns:a=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><a:mi>p<\/a:mi><a:mo>&lt;<\/a:mo><a:mn>0.001<\/a:mn><\/a:math><\/jats:inline-formula>) reduced AHT and heart rate. LME and LEAF significantly increased rat\u2019s body mass, plasmatic NO, and urinary creatinine and reduced urine NO and protein contents as compared to the L-NAME group. LME and its LEAF possess potent antihypertensive effects and further protect the renal function in L-NAME-induced hypertensive rats, thus supporting the use of C. zeylanicum in the management of AHT.<\/jats:p>","DOI":"10.1155\/2021\/2656249","type":"journal-article","created":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T20:50:05Z","timestamp":1625518205000},"page":"1-12","source":"Crossref","is-referenced-by-count":4,"title":["Antihypertensive Effects of the Methanol Extract and the Ethyl Acetate Fraction from Crinum zeylanicum (Amaryllidaceae) Leaves in L-NAME-Treated Rat"],"prefix":"10.1155","volume":"2021","author":[{"given":"Magloire Kanyou","family":"Ndjenda II","sequence":"first","affiliation":[{"name":"Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5754-7686","authenticated-orcid":true,"given":"Elvine Pami","family":"Nguelefack-Mbuyo","sequence":"additional","affiliation":[{"name":"Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert Donatien","family":"Atsamo","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Physiology, Faculty of Science, University of Yaound\u00e9 I, P.O. Box 812, Yaounde, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian Kuete","family":"Fofie","sequence":"additional","affiliation":[{"name":"Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chamberlin","family":"Fodem","sequence":"additional","affiliation":[{"name":"Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomain","family":"Nguemo","sequence":"additional","affiliation":[{"name":"Institute of Neurophysiology, University of Cologne, Cologne 50931, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5006-6927","authenticated-orcid":true,"given":"Telesphore Benoit","family":"Nguelefack","sequence":"additional","affiliation":[{"name":"Research Unit of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3238\/arztebl.2018.0557"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.4103\/0189-7969.165168"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1161\/hypertensionaha.120.15026"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.numecd.2005.11.012"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20143458"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2015.12.012"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22020209"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20092155"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1177\/1934578x19872931"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2020.04.029"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2018.08.087"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.4314\/njnpm.v1i1.11791"},{"issue":"39","key":"13","first-page":"1252","article-title":"Crinum; an endless source of bioactive principles: a review. part V. biological profile","volume":"4","author":"J. 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