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Angiotensin (Ang) IV possesses many biological properties that are not yet completely understood. Therefore, we investigated the function and mechanism of Ang IV in AMI in <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> conditions. AMI was performed by ligation of the left anterior descending coronary artery (LAD) in male C57 mice. Ang IV was continuously infused by a minipump 3\u2009d before AMI for 33\u2009d. The neonatal rat ventricular myocytes (NRVCs) were stimulated with Ang IV and cultured under hypoxic conditions. <jats:italic>In vivo<\/jats:italic>, Ang IV infusion significantly reduced the mortality after AMI. By the 7<jats:sup>th<\/jats:sup> day after AMI, compared with the AMI group, Ang IV reduced the inflammatory cytokine expression. Moreover, terminal deoxyribonucleotidyl transferase\u2010 (TDT\u2010) mediated dUTP nick\u2010end labeling (TUNEL) assay showed that Ang IV infusion reduced AMI\u2010induced cardiomyocyte apoptosis. Compared with AMI, Ang IV reduced autophagosomes in cardiomyocytes and improved mitochondrial swelling and disarrangement, as assessed by transmission electron microscopy. By 30<jats:sup>th<\/jats:sup> day after AMI, Ang IV significantly reduced the ratio of heart weight to body weight. Echocardiography showed that Ang IV improved impaired cardiac function. Hematoxylin and eosin (H&amp;E) and Masson staining showed that Ang IV infusion reduced the infarction size and myocardial fibrosis. <jats:italic>In vitro<\/jats:italic>, dihydroethidium (DHE) staining and comet assay showed that, compared with the hypoxia group, Ang IV reduced oxidative stress and DNA damage. Enzyme\u2010linked immunosorbent assay (ELISA) showed that Ang IV reduced hypoxia\u2010induced secretion of the tumor necrosis factor\u2010 (TNF\u2010) \u0251 and interleukin\u2010 (IL\u2010) 1<jats:italic>\u03b2<\/jats:italic>. In addition, compared with the hypoxia group, Ang IV reduced the transformation of light chain 3\u2010 (LC3\u2010) I to LC3\u2010II but increased p62 expression and decreased cardiomyocyte apoptosis. Overall, the present study showed that Ang IV reduced the inflammatory response, autophagy, and fibrosis after AMI, leading to reduced infarction size and improved cardiac function. Therefore, administration of Ang IV may be a feasible strategy for the treatment of AMI.<\/jats:p>","DOI":"10.1155\/2021\/2860488","type":"journal-article","created":{"date-parts":[[2021,12,14]],"date-time":"2021-12-14T23:50:06Z","timestamp":1639525806000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Continuous Infusion of Angiotensin IV Protects against Acute Myocardial Infarction via the Inhibition of Inflammation and 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