{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,31]],"date-time":"2025-12-31T07:48:22Z","timestamp":1767167302112,"version":"build-2238731810"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T00:00:00Z","timestamp":1633564800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672716"],"award-info":[{"award-number":["81672716"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81900571"],"award-info":[{"award-number":["81900571"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,10,7]]},"abstract":"<jats:p>Background. Hepatocellular carcinoma (HCC) is often diagnosed at a late stage, when the prognosis is poor. The regulation of long noncoding RNAs (lncRNAs) plays a crucial role in HCC. However, the precise regulatory mechanisms of lncRNA signaling in HCC remain largely unknown. Our study aims to investigate the underlying mechanisms of lncRNA (upregulated in hepatocellular carcinoma) URHC in HCC. Objective. To study the in vivo and in vitro localization and biological effects of URHC on liver cancer cells. Through bioinformatics analysis, dual-luciferase reporter gene analysis and rescue experiments revealed the possible mechanism of URHC. Methods. RT-qPCR, fluorescence in situ hybridization (FISH) staining, EdU, colony formation, and tumor xenograft experiments were used to identify localized and biological effects of URHC on HCC cells in vitro and in vivo. The bioinformatics analysis, dual-luciferase reporter assay, and rescue experiments revealed the potential mechanism of URHC. Results. URHC silencing may inhibit the HCC cells\u2019 proliferation in vitro and in vivo. We found that URHC was mainly localized in the cytoplasm. The expression of miR-5007-3p was negatively regulated by URHC. And miR-5007-3p could reverse the effect of URHC in HCC cells. The expression of DNAJB9 was negatively regulated by miR-5007-3p but positively regulated by URHC. These suggestive of lncRNA-URHC positively regulated the level of DNAJB9 by sponging miR-5007-3p. Conclusion. Together, our study elucidated the role of URHC as a miRNA sponge in HCC and shed new light on lncRNA-directed diagnostics and therapeutics in HCC.<\/jats:p>","DOI":"10.1155\/2021\/3031482","type":"journal-article","created":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T13:57:00Z","timestamp":1633874220000},"page":"1-13","source":"Crossref","is-referenced-by-count":1,"title":["LncRNA-URHC Functions as ceRNA to Regulate DNAJB9 Expression by Competitively Binding to miR-5007-3p in Hepatocellular Carcinoma"],"prefix":"10.1155","volume":"2021","author":[{"given":"Kunwei","family":"Niu","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle Road, Xi\u2019an, Shaanxi 710032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shibin","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle Road, Xi\u2019an, Shaanxi 710032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle Road, Xi\u2019an, Shaanxi 710032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jimin","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle Road, Xi\u2019an, Shaanxi 710032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianlin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle Road, Xi\u2019an, Shaanxi 710032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Nie","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle Road, Xi\u2019an, Shaanxi 710032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle Road, Xi\u2019an, Shaanxi 710032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8573-0445","authenticated-orcid":true,"given":"Kaishan","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle Road, Xi\u2019an, Shaanxi 710032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6628-7302","authenticated-orcid":true,"given":"Wenjie","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, 127 Changle Road, Xi\u2019an, Shaanxi 710032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1038\/s41389-018-0097-8"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1186\/s13148-018-0570-4"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1080\/136518201753242262"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/7526042"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-018-0579-3"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2014.941285"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncrna.2017.11.001"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26018"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-017-2370-1"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1159\/000495231"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1159\/000493445"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq285"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2015.08.049"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-017-4508-z"},{"issue":"9","key":"15","first-page":"15591","article-title":"Biological significance of long non-coding RNA FTX expression in human colorectal cancer","volume":"8","author":"X. 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