{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:40:41Z","timestamp":1791916841875,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Construction of Key Disciplines of Traditional Chinese Medicine from Health Commission of Pudong New Area","award":["PDZY-2018-0608"],"award-info":[{"award-number":["PDZY-2018-0608"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,12,1]]},"abstract":"<jats:p>Sheng Jing Decoction (SJD), as a traditional Chinese medicine prescription, is mainly be used to treat male infertility. However, the pharmacological functions and molecular mechanisms of SJD are poorly understood. In this study, we investigated the functions of SJD on spermatogenesis and sperm motility and explored the potential mechanisms involved. Here, we demonstrated that high, medium, and low doses of SJD are effective in restoring the impairments of the whole body and testicular tissue by cyclophosphamide inducing and to rescue the damage of testicular tissue cells including Sertoli cells and germ cells. SJD can partly restore the decrease in sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate in oligozoospermic mouse models. Ki67 staining analyses confirm SJD can promote testicular tissue cell proliferation. Real-time RT-PCR analyses also reveal that SJD can upregulate the expression of proliferation-associated gene Lin28a and differentiation-associated genes Kit, Sohlh2, and Stra8. SJD can also reduce the impairment of mitochondrial membrane potential (MMP) and sperm plasma membrane integrity by cyclophosphamide inducing. Our results reveal that SJD is effective in improving both sperm quantity and quality by increasing the sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate. SJD can promote spermatogenesis by upregulating the expression of the proliferation-associated gene Lin28a and the differentiation-associated genes (Kit, Sohlh2, and Stra8). SJD can sustain MMP and sperm plasma membrane integrity to increase sperm motility.<\/jats:p>","DOI":"10.1155\/2021\/3686494","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T17:50:10Z","timestamp":1638381010000},"page":"1-9","source":"Crossref","is-referenced-by-count":5,"title":["Sheng Jing Decoction Can Promote Spermatogenesis and Increase Sperm Motility of the Oligozoospermia Mouse Model"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6784-0010","authenticated-orcid":true,"given":"Guang","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Urology and Reproductive Medicine, Seventh People\u2019s Hospital of Shanghai University of TCM, Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3897-0999","authenticated-orcid":true,"given":"Fang","family":"Tian","sequence":"additional","affiliation":[{"name":"NHC Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), Fudan University, Shanghai 200032, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4048-0999","authenticated-orcid":true,"given":"Peng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology and Reproductive Medicine, Seventh People\u2019s Hospital of Shanghai University of TCM, Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0682-5324","authenticated-orcid":true,"given":"Jianming","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Urology and Reproductive Medicine, Seventh People\u2019s Hospital of Shanghai University of TCM, Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-596X","authenticated-orcid":true,"given":"Jianmin","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Urology and Reproductive Medicine, Seventh People\u2019s Hospital of Shanghai University of TCM, Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1489-8758","authenticated-orcid":true,"given":"Wenjun","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Urology and Reproductive Medicine, Seventh People\u2019s Hospital of Shanghai University of TCM, Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7875-2235","authenticated-orcid":true,"given":"Ran","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Urology and Reproductive Medicine, Seventh People\u2019s Hospital of Shanghai University of TCM, Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7351-1969","authenticated-orcid":true,"given":"Shishuai","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Urology and Reproductive Medicine, Seventh People\u2019s Hospital of Shanghai University of TCM, Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4419-9034","authenticated-orcid":true,"given":"Quanyao","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Urology and Reproductive Medicine, Seventh People\u2019s Hospital of Shanghai University of TCM, Shanghai 200137, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001356"},{"key":"2","volume-title":"WHO Manual for the Standardized Investigation, Diagnosis and Management of the Infertile Male","author":"P. 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