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Increasing evidence is supporting the potential application of natural resourced polysaccharides as promising prevention or treatment strategies against NAFLD. In the current study, an acidic heteropolysaccharide, LFP\u2010a1, was isolated and purified from Lycii fructus with successively hot water refluxing extraction, alcohol precipitation, protein removal, and DEAE\u201052 cellulose chromatographic separation. LFP\u2010a1 was a complicated structured polysaccharide with an average MW of 4.74 \u00d7 104\u2009Da and composed of 6 monosaccharides and 1 uronic acid. Preexposure of LFP\u2010a1 could increase the cell viability and reverse the abnormal oxidative stress though inhibition of mitochondrial\u2010mediated apoptotic pathway and correction of cell cycle progression against H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> hepatoxicity in NAFLD model L02 cells. Consistently, <jats:italic>in vivo<\/jats:italic> study in thioacetamide\u2010 (TAA\u2010) induced NAFLD model zebrafish larvae showed LFP\u2010a1 preserved the liver integrity and alleviated TAA\u2010induced oxidative stress through downregulation of abnormal apoptosis. These observations indicated the hepatoprotective activity of LFP\u2010a1, which may be applied for the prevention or treatment of NAFLD or other oxidative stress\u2010related diseases.<\/jats:p>","DOI":"10.1155\/2021\/3923625","type":"journal-article","created":{"date-parts":[[2021,2,19]],"date-time":"2021-02-19T00:36:39Z","timestamp":1613694999000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Hepatoprotection of Lycii Fructus Polysaccharide against Oxidative Stress in Hepatocytes and Larval 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