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However, the mechanisms underlying the disease remain unclear. microRNAs (miRNAs) have been indicated to play a crucial role in the development of NRDS. In this study, we aimed to explore the regulatory mechanisms of miR\u2010296\u20105p in NRDS. The expression levels of miR\u2010296\u20105p in preterm infants with NRDS were determined using quantitative reverse\u2010transcription polymerase chain reaction (RT\u2010qPCR). A549 cells were transfected with lentiviral vectors encoding miR\u2010296\u20105p, and the transfection efficiency was determined using RT\u2010qPCR. Flow cytometry and CCK8 assay were performed to measure apoptosis and proliferation of A549 cells, respectively. The protein levels of pulmonary surfactant SP\u2010A (SFTPA1), SP\u2010B, Wnt7b, and <jats:italic>\u03b2<\/jats:italic>\u2010catenin were measured using western blotting. We demonstrated an upregulation of miR\u2010296\u20105p in NRDS. The miR\u2010296\u20105p was successfully overexpressed in A549 cells via lentivirus transfection, and the upregulation of miR\u2010296\u20105p inhibited cell proliferation and secretion of SP\u2010A and SP\u2010B and also induced downregulation of the Wnt7b\/<jats:italic>\u03b2<\/jats:italic>\u2010catenin <jats:italic>in vitro<\/jats:italic>. Therefore, miR\u2010296\u20105p inhibits cell proliferation and secretion of pulmonary surfactants in A549 cells via downregulation of Wnt7b\/<jats:italic>\u03b2<\/jats:italic>\u2010catenin signaling.<\/jats:p>","DOI":"10.1155\/2021\/4051504","type":"journal-article","created":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T21:37:05Z","timestamp":1610401025000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["miR\u2010296\u20105p Inhibits the Secretion of Pulmonary Surfactants in Pulmonary Epithelial Cells via the Downregulation of Wnt7b\/<i>\u03b2<\/i>\u2010Catenin Signaling"],"prefix":"10.1155","volume":"2021","author":[{"given":"Ying-Hui","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai-Ling","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren-Qiang","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei-Bei","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan-Ni","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Zi","family":"Mei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang-Dong","family":"Fang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan-Yu","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1741-0968","authenticated-orcid":false,"given":"Bing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,5]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1159\/000448985"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1953310"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3074"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg.2016.134"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prrv.2016.12.002"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.21047"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.90268.2008"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0010854"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510839103"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.08.007"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1139253"},{"key":"e_1_2_10_12_2","first-page":"1302","article-title":"Prediction of microRNA-296-5p target genes and its application in lung development","volume":"18","author":"Zhang Y. 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