{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T14:46:16Z","timestamp":1790606776880,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T00:00:00Z","timestamp":1623196800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771646"],"award-info":[{"award-number":["81771646"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81701506"],"award-info":[{"award-number":["81701506"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673984"],"award-info":[{"award-number":["81673984"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074444"],"award-info":[{"award-number":["82074444"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["45835"],"award-info":[{"award-number":["45835"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["81771646"],"award-info":[{"award-number":["81771646"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["81701506"],"award-info":[{"award-number":["81701506"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["81673984"],"award-info":[{"award-number":["81673984"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["82074444"],"award-info":[{"award-number":["82074444"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["45835"],"award-info":[{"award-number":["45835"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2021,6,9]]},"abstract":"<jats:p>To investigate the effect and mechanism of action of Moriamin Forte (MF) on oligoasthenospermia (OA) in rats exposed to multiglycosides of Tripterygium wilfordii (GTW), forty male Sprague Dawley rats were randomly divided into four groups. Rats in the control group were treated with 0.5% sodium carboxymethyl cellulose. The remaining rats were administered GTW (30\u2009mg\/kg\/d) for 40\u2009d to establish an OA model. Concurrently, the groups were treated with normal saline and low-dose (100\u2009mg\/kg\/d) and high-dose (200\u2009mg\/kg\/d) MF, respectively. After treatment, the number and motility of sperm cells were examined. Testicular and epididymal histomorphology changes were observed. Antioxidant indicators (SOD, CAT, MDA, TAC, and Nrf2) in testicular and epididymal tissues were detected. Apoptotic and antiapoptotic indicators (Bax and Bcl2 expression) in the testicular tissue were measured by immunohistochemistry. GTW decreased sperm count and motility, damaged testicular and epididymis tissues, impaired antioxidase activity, and increased tissue MDA levels. Meanwhile, GTW upregulated the expression of Bax and downregulated the expression of Bcl2. Western blot analysis demonstrated a decrease in the Nrf2 expression in the model group. Treatment with MF improved sperm count and motility, as well as inhibited the rate of apoptosis in the rat reproductive system. Moreover, MF improved the activity of antioxidants and increased the relative expression of the antioxidant pathway-related protein Nrf2. In conclusion, MF may reverse the GTW-induced OA by modulating the expression of apoptotic and antioxidant pathway-related proteins. This study may provide a pharmacological foundation for the use of MF in OA treatment.<\/jats:p>","DOI":"10.1155\/2021\/4059248","type":"journal-article","created":{"date-parts":[[2021,6,9]],"date-time":"2021-06-09T19:06:18Z","timestamp":1623265578000},"page":"1-9","source":"Crossref","is-referenced-by-count":2,"title":["Impact of Moriamin Forte on Testicular and Epididymal Damage in Rats with Oligoasthenospermia"],"prefix":"10.1155","volume":"2021","author":[{"given":"Xing","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Andrology, Jinling Hospital Affiliated to Nanjing University School of Medicine, No. 305, East Zhongshan Road, Nanjing, Jiangsu 210002, China"},{"name":"Department of Andrology, The First Affiliated Hospital of Hunan University of Chinese Medicine, No. 95, Shaoshan Middle Road, Changsha, Hunan 410007, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology, Suqian First Hospital, No. 120, Suzhi Road, Suqian, Jiangsu 223800, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Ning","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Andrology, Jinling Hospital Affiliated to Southern Medical University, No. 305, East Zhongshan Road, Nanjing, Jiangsu 210002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ze","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Andrology, Jinling Hospital Affiliated to Southern Medical University, No. 305, East Zhongshan Road, Nanjing, Jiangsu 210002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Song","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Urology, General Hospital of Eastern Theater Command, No. 305, East Zhongshan Road, Nanjing, Jiangsu 210002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling-Ling","family":"Yan","sequence":"additional","affiliation":[{"name":"Laboratory of Reproductive Medicine, Shenzhen Wanhe Enterprise Technology Center, 12\/F, Block C, Wanhe Technology Complex, 7 Huitong Road, Guangming District, Shenzhen, Guangdong 518107, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Jun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Dongcheng District, Beijing 100730, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9402-1464","authenticated-orcid":true,"given":"Xue-Jun","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of Andrology, Jinling Hospital Affiliated to Nanjing University School of Medicine, No. 305, East Zhongshan Road, Nanjing, Jiangsu 210002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","volume-title":"WHO Laboratory Manual for the Examination and Processing of Human Semen","author":"World Health Organization","year":"2010","edition":"5th"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2012.04.048"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.2164\/jandrol.108.007278"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1111\/andr.12336"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2016.12.008"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.26.9.3610-3624.2006"},{"issue":"9","key":"7","first-page":"571","article-title":"Advances in research of triptolide-induced impaired male reproductive function","volume":"28","author":"F. Li","year":"2008","journal-title":"Reproduction & Contraception"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.21010\/ajtcam.v14i4.11"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3390\/nu11040862"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1093\/tropej\/fmz059"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2019.06.010"},{"key":"12","volume-title":"Vitamin B1 Thiamine Deficiency (Beriberi)","author":"K. D. Wiley","year":"2019"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.12968\/bjon.2018.27.12.661"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1530\/eje-19-0197"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8060830"},{"issue":"5","key":"16","first-page":"365","article-title":"Clinical efficacy of compound amino acids capsules (8\u201311) for physical rehabilitation following artificial abortion","volume":"20","author":"Z. Z. Wu","year":"2009","journal-title":"China Pharmacy"},{"issue":"2","key":"17","first-page":"61","article-title":"Exploring the human seminal plasma proteome: an unexplored gold mine of biomarker for male infertility and male reproduction disorder","volume":"16","author":"K. Gilany","year":"2015","journal-title":"Journal of reproduction & infertility"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1111\/and.12841"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2016.03.007"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.10.017"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2014.10.005"},{"issue":"5","key":"22","first-page":"336","article-title":"Effects of semen extender supplemented with L-methionine and packaging methods (straws and pellets) on postithaw goldfish (Carassius auratus) sperm quality and DNA damage","volume":"36","author":"F. Kutluyer","year":"2015","journal-title":"Cryo Letters"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.2174\/138920011796504536"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2014.01.011"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.2174\/1568026023394443"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.4081\/aiua.2014.3.164"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/s0300-483x(02)00380-3"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1042\/bj0900060"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01575.x"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.4103\/0974-1208.63115"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2014\/8432.4622"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2016.09.023"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1111\/and.12959"},{"issue":"5","key":"34","first-page":"396","article-title":"Strontium fructose 1,6-diphosphate (FDP-Sr) alleviates oligozoospermia induced by multiglycosides of Tripterygium wilfordii in rats","volume":"17","author":"Y. Y Hu","year":"2011","journal-title":"Zhonghua Nan Ke Xue"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-015-1059-0"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1055\/s-0031-1300555"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2019.104639"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.anireprosci.2010.01.010"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8090353"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.13220\/j.cnki.jipr.2016.01.022"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1097\/mco.0b013e32834136f2"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1072"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1007387107"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2017.95"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/4059248.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/4059248.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2021\/4059248.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,26]],"date-time":"2022-10-26T09:57:41Z","timestamp":1666778261000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ecam\/2021\/4059248\/"}},"subtitle":[],"editor":[{"given":"Vincenzo","family":"De Feo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,6,9]]},"references-count":44,"alternative-id":["4059248","4059248"],"URL":"https:\/\/doi.org\/10.1155\/2021\/4059248","relation":{},"ISSN":["1741-4288","1741-427X"],"issn-type":[{"value":"1741-4288","type":"electronic"},{"value":"1741-427X","type":"print"}],"subject":[],"published":{"date-parts":[[2021,6,9]]}}}