{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:26:08Z","timestamp":1792103168970,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T00:00:00Z","timestamp":1639353600000},"content-version":"vor","delay-in-days":346,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001501","name":"University Grants Commission","doi-asserted-by":"publisher","award":["MRS-77\/78-S&T-29"],"award-info":[{"award-number":["MRS-77\/78-S&T-29"]}],"id":[{"id":"10.13039\/501100001501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>\u03b1<\/jats:italic>\u2010Amylase is an enzyme involved in the breaking down of large insoluble starch molecules into smaller soluble glucose molecules. <jats:italic>Catunaregam spinosa<\/jats:italic> (Thunb.) Tirveng. (syn. <jats:italic>Randia dumetorum<\/jats:italic> (Retz.) Lam., Family: Rubiaceace) has been used as traditional medicine for the treatment of gastrointestinal problems, skin diseases, and diabetes. In this context, we studied the <jats:italic>in vitro \u03b1<\/jats:italic>\u2010amylase inhibiting properties of methanol extracts of leaves and bark of <jats:italic>C. spinosa<\/jats:italic>. The methanol extract of bark was further fractionated into hexane, dichloromethane and ethyl acetate, and water\u2010soluble fractions, and their <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibitory activity was evaluated. <jats:italic>In silico<\/jats:italic> molecular docking and ADMET analysis of several compounds previously reported from the bark of <jats:italic>C. spinosa<\/jats:italic> were also performed. The <jats:italic>in vitro \u03b1<\/jats:italic>\u2010amylase inhibition activity assay of the dichloromethane fraction of extract of bark (IC<jats:sub>50<\/jats:sub>: 77.17 \u00b1 1.75\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) was more potent as compared to hexane and ethyl acetate fractions. The <jats:italic>in silico<\/jats:italic> molecular docking study showed that previously reported compounds from the stem bark such as balanophonin, catunaregin, <jats:italic>\u03b2<\/jats:italic>\u2010sitosterol, and medioresinol were bounded well with the active catalytic residue of porcine pancreatic <jats:italic>\u03b1<\/jats:italic>\u2010amylase indicating better inhibition. The ADMET analysis showed the possible drug\u2010likeness and structure\u2010activity relationship of selected compounds. These compounds should be studied further for their potential <jats:italic>\u03b1<\/jats:italic>\u2010amylase inhibition in animal models.<\/jats:p>","DOI":"10.1155\/2021\/4133876","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T21:20:13Z","timestamp":1639430413000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["<i>\u03b1<\/i>\u2010Amylase Inhibitory Activity of <i>Catunaregam spinosa<\/i> (Thunb.) Tirveng.: <i>In Vitro<\/i> and <i>In Silico<\/i> Studies"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2124-8809","authenticated-orcid":false,"given":"Deepak","family":"Timalsina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9155-2799","authenticated-orcid":false,"given":"Deepti","family":"Bhusal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0509-1621","authenticated-orcid":false,"given":"Hari Prasad","family":"Devkota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8213-6416","authenticated-orcid":false,"given":"Krishna Prasad","family":"Pokhrel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-0887","authenticated-orcid":false,"given":"Khaga Raj","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,12,13]]},"reference":[{"key":"e_1_2_11_1_2","first-page":"14","article-title":"Purification and characterization of 0-amylase from","volume":"3","author":"Afifi A. 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