{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T17:48:24Z","timestamp":1777052904575,"version":"3.51.4"},"reference-count":65,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T00:00:00Z","timestamp":1633651200000},"content-version":"vor","delay-in-days":280,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004413","name":"International Foundation for Science","doi-asserted-by":"publisher","award":["F\/5495-2"],"award-info":[{"award-number":["F\/5495-2"]}],"id":[{"id":"10.13039\/100004413","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Stephania abyssinica<\/jats:italic> is a medicinal plant used in Cameroon alternative medicine to treat arterial hypertension (AHT). Previous <jats:italic>in vitro<\/jats:italic> studies demonstrated the endothelium nitric oxide\u2010independent vasorelaxant property of the aqueous extract from <jats:italic>Stephania abyssinica<\/jats:italic> (AESA). But its effect on AHT is unknown. The present study was undertaken to explore other vasorelaxant mechanisms and to determine the antihypertensive effects of AESA in male Wistar rats. Phytochemical analysis of AESA was carried out using the liquid chromatography\u2010mass spectrometry (LC\u2010MS) method. The vasorelaxant effects of AESA (1\u20101000\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) were studied on rat isolated thoracic aorta rings, in the absence or presence of indomethacin (10\u2009<jats:italic>\u03bc<\/jats:italic>M) or methylene blue (10\u2009<jats:italic>\u03bc<\/jats:italic>M). The inhibitory effect of AESA on phenylephrine (PE, 10\u2009<jats:italic>\u03bc<\/jats:italic>M) or KCl\u2010 (60\u2009mM) induced contraction as well as the intracellular calcium release was also evaluated. The <jats:italic>in vivo<\/jats:italic> antihypertensive activity of AESA (43, 86, or 172\u2009mg\/kg\/day) or captopril (20\u2009mg\/kg\/day) administered orally was assessed in L\u2010NAME\u2010 (40\u2009mg\/kg\/day) treated rats. Blood pressure and heart rate (HR) were measured at the end of each week while serum or urinary nitric oxide (NO), creatinine, and glomerular filtration rate (GFR) were determined at the end of the 6 weeks of treatment, as well as histological analysis of the heart and the kidney. The LC\u2010MS profiling of AESA identified 9 compounds including 7 alkaloids. AESA produced a concentration\u2010dependent relaxation on contraction induced either by PE and KCl, which was significantly reduced in endothelium\u2010denuded vessels, as well as in vessels pretreated with indomethacin and methylene blue. Moreover, AESA inhibited the intracellular Ca<jats:sup>2+<\/jats:sup> release\u2010induced contraction. In vivo, AESA reduced the AHT, heart rate (HR), and ventricular hypertrophy and increased serum NO, urine creatinine, and GFR. AESA also ameliorated heart and kidney lesions as compared to the L\u2010NAME group. These findings supported the use of AESA as a potential antihypertensive drug.<\/jats:p>","DOI":"10.1155\/2021\/4730341","type":"journal-article","created":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T22:58:02Z","timestamp":1633906682000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Vasorelaxant\u2010Mediated Antihypertensive Effect of the Leaf Aqueous Extract from <i>Stephania abyssinica<\/i> (Dillon &amp; A. Rich) Walp (Menispermaceae) in Rat"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2135-7263","authenticated-orcid":false,"given":"Chamberlin","family":"Fodem","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5754-7686","authenticated-orcid":false,"given":"Elvine Pami","family":"Nguelefack-Mbuyo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8752-543X","authenticated-orcid":false,"suffix":"II","given":"Magloire Kanyou","family":"Ndjenda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0657-406X","authenticated-orcid":false,"given":"Albert","family":"Kamanyi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5006-6927","authenticated-orcid":false,"given":"T\u00e9lesphore Benoit","family":"Nguelefack","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,8]]},"reference":[{"key":"e_1_2_11_1_2","first-page":"103","article-title":"Cameroon country report PASCAR and WHF","volume":"31","author":"Dzudie A.","year":"2020","journal-title":"Cardiovascular Diseases Scorecard Project"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.120.318729"},{"key":"e_1_2_11_3_2","volume-title":"High systolic blood pressure-level 2 risk","author":"Institute for Health Metrics and Evaluation","year":"2019"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.115.018912"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-019-4431-9"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000000596"},{"key":"e_1_2_11_7_2","doi-asserted-by":"crossref","unstructured":"OkelloS. 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