{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:17:42Z","timestamp":1790957862634,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,12,11]],"date-time":"2021-12-11T00:00:00Z","timestamp":1639180800000},"content-version":"vor","delay-in-days":344,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Superenhancer usages in single cancer form such as colorectal cancer (CRC) may provide novel efficient targeting candidates. It is unclear whether CRC contains recurrent superenhancers that confer a predisposition to malignancy. We investigated the superenhancer profile of CRC cell line HCT116 and compared it to that of a healthy sigmoid colon. We found that HCT116 had lost most of the normal colon superenhancer activities but gained a new set of tumor\u2010favoring superenhancers that facilitate tumor proliferation, growth signalling, and hypoxia resistance. Inhibiting the superenhancers by JQ\u20101 treatment had significantly decreased the colony formation capability of HCT116. Then, by comparing the superenhancer genes and robust CRC upregulated genes, we identified a superenhancer associated with a common CRC upregulated oncogene, <jats:italic>POU5f1B<\/jats:italic>. <jats:italic>POU5f1B<\/jats:italic> overexpression is related to the worse outcome in CRCs. Via performing ChIP\u2010PCR in 35 clinical samples and investigating CRC anti\u2010H3K27ac ChiP\u2010seq public dataset consisting of 36 samples, we further identified that the superenhancer of oncogene <jats:italic>POU5F1B<\/jats:italic> is recurrently activated in CRCs, taking 62 and 72 per cent, respectively. Moreover, JQ\u20101 treatment successfully inhibited the POU5F1B expression in 5 out of 6 <jats:italic>POU5F1B<\/jats:italic> superenhancer\u2010positive samples. Therefore, we concluded that the superenhancer activation of <jats:italic>POU5F1B<\/jats:italic> contributes partially to its high expression in CRCs, in addition to the well\u2010known gene amplification aetiology.<\/jats:p>","DOI":"10.1155\/2021\/5405060","type":"journal-article","created":{"date-parts":[[2021,12,11]],"date-time":"2021-12-11T22:05:09Z","timestamp":1639260309000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Recurrent Superenhancer of the Oncogene <i>POU5F1B<\/i> in Colorectal 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