{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:19:47Z","timestamp":1753885187845,"version":"3.41.2"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T00:00:00Z","timestamp":1624924800000},"content-version":"vor","delay-in-days":179,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Autophagy is likely altered in patients with endometriosis. Ovarian steroid hormones seem to affect this changing of the autophagic process. <jats:italic>Objective<\/jats:italic>. To study the effect of combined oral contraceptive (COC) pills on the expression of autophagic\u2010related gene <jats:italic>BECN1<\/jats:italic> and <jats:italic>LC3B<\/jats:italic> in the ectopic and eutopic endometria of patients with endometriosis. <jats:italic>Material and Methods<\/jats:italic>. The present quasiexperimental study recruited 36 women (18\u201345 years old) with endometrioma and nonendometrioma who were scheduled for surgery. Patients with endometrioma were randomly assigned to either a no\u2010treatment group (<jats:italic>n<\/jats:italic> = 12) or a COC group (<jats:italic>n<\/jats:italic> = 12). The COC group was prescribed a daily oral pill composed of 3\u2009mg drospirenone and 0.03\u2009mg ethinyl estradiol for 6 weeks before surgery. The control group (<jats:italic>n<\/jats:italic> = 12) was composed of women without endometrioma. Ectopic endometriotic and endometrium tissues were collected from the no\u2010treatment and COC groups, whereas the only endometrium was collected from the control group. These tissues were used for real\u2010time PCR to measure the expression of the <jats:italic>BECN1<\/jats:italic> and <jats:italic>LC3B<\/jats:italic> genes. <jats:italic>Results<\/jats:italic>. The baseline demographic data were not different among the three groups. The <jats:italic>BECN1<\/jats:italic> gene expression in endometrium tissue in the COC group was significantly less than that in the no\u2010treatment and control groups (<jats:italic>P<\/jats:italic> = 0.011 and 0.029, respectively). No significant difference of endometriotic cyst <jats:italic>BECN1<\/jats:italic> and <jats:italic>LC3B<\/jats:italic> gene expression was found between COC and no treatment. <jats:italic>Conclusions<\/jats:italic>. Oral COC pills for 6 weeks continuously before surgery decreased the eutopic endometrial expression (mRNA) of the <jats:italic>BECN1<\/jats:italic> gene compared to those from healthy normal women and nontreated patients with an endometriotic cyst. The change in the expression of autophagy\u2010related genes was more distinct in eutopic than ectopic endometria. This trial is registered with <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/www.thaiclinicaltrials.org\">TCTR20170720002<\/jats:ext-link>. Registered and enrolled the first patient on 20 July 2017.<\/jats:p>","DOI":"10.1155\/2021\/5519538","type":"journal-article","created":{"date-parts":[[2021,6,29]],"date-time":"2021-06-29T18:20:10Z","timestamp":1624990810000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Effect of Combined Oral Contraceptive Pills on Beclin\u20101 and LC3B Transcript Levels in Ovarian Endometrioma"],"prefix":"10.1155","volume":"2021","author":[{"given":"Wanwisa","family":"Waiyaput","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ongarj","family":"Bovornsakulvong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srithean","family":"Lertvikool","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4589-0030","authenticated-orcid":false,"given":"Areepan","family":"Sophonsritsuk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,6,29]]},"reference":[{"key":"e_1_2_10_1_2","first-page":"93","article-title":"Metastatic or embolic endometriosis, due to the menstrual dissemination of endometrial tissue into the venous circulation","volume":"3","author":"Sampson J. 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