{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:56:46Z","timestamp":1792112206651,"version":"4.4.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T00:00:00Z","timestamp":1629417600000},"content-version":"vor","delay-in-days":231,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82004001"],"award-info":[{"award-number":["82004001"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82071008"],"award-info":[{"award-number":["82071008"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    Age\u2010related macular degeneration (AMD) is a common and severe blinding disease among people worldwide. Retinal inflammation and neovascularization are two fundamental pathological processes in AMD. Recent studies showed that P2X7 receptor was closely involved in the inflammatory response. Here, we aim to investigate whether A740003, a P2X7 receptor antagonist, could prevent retinal inflammation and neovascularization induced by oxidized low\u2010density lipoprotein (ox\u2010LDL) and explore the underlying mechanisms. ARPE\u201019 cells and C57BL\/6 mice were treated with ox\u2010LDL and A740003 successively for i\n                    <jats:italic>n vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    studies. In this research, we found that A740003 suppressed reactive oxygen species (ROS) generation and inhibited the activation of Nod\u2010like receptor pyrin\u2010domain protein 3 (NLRP3) inflammasome and nuclear factor\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B (NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B) pathway. A740003 also inhibited the generation of angiogenic factors in ARPE\u201019 cells and angiogenesis in mice. The inflammatory cytokines and phosphorylation of inhibitor of nuclear factor\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B alpha (IKB\n                    <jats:italic>\u03b1<\/jats:italic>\n                    ) were repressed by A740003. Besides, ERG assessment showed that retinal functions were remarkably preserved in A740003\u2010treated mice. In summary, our results revealed that the P2X7 receptor antagonist reduced retinal inflammation and neovascularization and protected retinal function. The protective effects were associated with regulation of NLRP3 inflammasome and the NF\u2010\n                    <jats:italic>\u03ba<\/jats:italic>\n                    B pathway, as well as inhibition of angiogenic factors.\n                  <\/jats:p>","DOI":"10.1155\/2021\/5520644","type":"journal-article","created":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T18:19:42Z","timestamp":1629483582000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["P2X7 Receptor Antagonist Attenuates Retinal Inflammation and Neovascularization Induced by Oxidized Low\u2010Density Lipoprotein"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4121-6620","authenticated-orcid":false,"given":"Mingzhu","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1854-0577","authenticated-orcid":false,"given":"Ruiqi","family":"Qiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2171-5625","authenticated-orcid":false,"given":"Weiping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1579-825X","authenticated-orcid":false,"given":"Jingyang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2978-303X","authenticated-orcid":false,"given":"Xiuxiu","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8253-4932","authenticated-orcid":false,"given":"Ya","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6888-7571","authenticated-orcid":false,"given":"Lei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5497-0905","authenticated-orcid":false,"given":"Bo","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,20]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.4266"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165653"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10238-004-0054-z"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/aos.13380"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1212660"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2015.1017183"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0074"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05211.x"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.06-0699"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0b013e31822529b1"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1677\/JME-08-0011"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/690243"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20092101"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2019.07.010"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuropharm.2015.05.005"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00123"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21239305"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2011.01.013"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2017.05.001"},{"key":"e_1_2_10_20_2","first-page":"188","article-title":"Osmotic 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