{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T02:15:20Z","timestamp":1793153720720,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,5,17]],"date-time":"2021-05-17T00:00:00Z","timestamp":1621209600000},"content-version":"vor","delay-in-days":136,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970676"],"award-info":[{"award-number":["81970676"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Diabetes mellitus is a clinical syndrome caused by genetic and environmental factors. Growing evidence suggests that exposure to environmental endocrine disruptors and activation of NLRP3 inflammasome signaling play a vital role in diabetes. However, it is unclear how PCB118, a common environmental endocrine disruptor, contributes to the incidence of diabetes, and its specific mechanism of action is unknown. In this study, we explored whether ROS\u2010induced NLRP3 inflammasome priming and activation were related to PCB118 exposure in mouse islet <jats:italic>\u03b2<\/jats:italic>\u2010TC\u20106 cells and the mechanisms of diabetes. <jats:italic>Methods<\/jats:italic>. Mouse islet <jats:italic>\u03b2<\/jats:italic>\u2010TC\u20106 cells were cultured with PCB118 as a stimulating factor and ROS inhibitor N\u2010acetyl cysteine (NAC) as an intervention. Cellular toxicity due to PCB118 was detected using the Cell Counting Kit\u20108; ROS was measured using DCFH\u2010DA; the expressions of NLRP3, procaspase\u20101, caspase\u20101, pro\u2010IL\u20101<jats:italic>\u03b2<\/jats:italic>, and IL\u20101<jats:italic>\u03b2<\/jats:italic> protein were detected by western blot; and IL\u20106, IL\u201018, and C\u2010C chemokine ligand 2 (CCL\u20102) were measured by ELISA. <jats:italic>Results<\/jats:italic>. PCB118 caused significant toxicity to the cells when the stimulation concentration was equal to or greater than 80\u2009nmol\/L at 72 hours (<jats:italic>p<\/jats:italic> &lt; 0.05) and increased the levels of ROS, NLRP3, caspase\u20101, IL\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20106, IL\u201018, and CCL\u20102 (<jats:italic>p<\/jats:italic> &lt; 0.05); the expressions of procaspase\u20101 and pro\u2010IL\u20101<jats:italic>\u03b2<\/jats:italic> were downregulated in a dose\u2010dependent manner after PCB118 exposure (<jats:italic>p<\/jats:italic> &lt; 0.05), which was prevented by pretreatment with NAC (<jats:italic>p<\/jats:italic> &lt; 0.05). <jats:italic>Conclusions<\/jats:italic>. PCB118 can activate NLRP3 inflammasome signaling in islet beta cells via the oxidative stress pathway and cause inflammation in islet beta cells. It suggests that environmental endocrine disruptors play an important role in the inflammation of islet beta cells and may contribute to the development of diabetes through NLRP3 inflammatory signaling.<\/jats:p>","DOI":"10.1155\/2021\/5522578","type":"journal-article","created":{"date-parts":[[2021,5,18]],"date-time":"2021-05-18T00:20:09Z","timestamp":1621297209000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["PCB118 Induces Inflammation of Islet Beta Cells via Activating ROS\u2010NLRP3 Inflammasome Signaling"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3880-9388","authenticated-orcid":false,"given":"Chunxia","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6419-1983","authenticated-orcid":false,"given":"Yuping","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7646-669X","authenticated-orcid":false,"given":"Man","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4668-0681","authenticated-orcid":false,"given":"Yang","family":"Long","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3414-1170","authenticated-orcid":false,"given":"Jiao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0836-2386","authenticated-orcid":false,"given":"Fang","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9373-8746","authenticated-orcid":false,"given":"Shi","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9534-6252","authenticated-orcid":false,"given":"Yong","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,17]]},"reference":[{"key":"e_1_2_10_1_2","unstructured":"International Diabetes Federation IDF diabetes atlas 9th edn. 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