{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T22:19:25Z","timestamp":1785449965801,"version":"3.56.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T00:00:00Z","timestamp":1622160000000},"content-version":"vor","delay-in-days":147,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Stein Erik Hagens Foundation for Clinical Heart Research, Oslo, Norway"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. The Nod\u2010Like\u2010Receptor\u2010Protein\u20103 (NLRP3) inflammasome and the Interleukin\u20106 (IL\u20106) pathways are central mechanisms of the inflammatory response in myocardial reperfusion injury. Expanding our knowledge about the inflammasome signaling axis is important to improve treatment options. In a cross\u2010sectional study, we aimed to study presence, localization, and genetic expression of inflammasome\u2010 and IL\u20106\u2010 signaling\u2010related proteins in coronary thrombi and circulating leukocytes from ST\u2010elevation myocardial infarction (STEMI) patients, with relation to myocardial injury and time from symptoms to PCI. <jats:italic>Methods<\/jats:italic>. Intracoronary thrombi were aspirated from 33 STEMI patients. Blood samples were drawn. mRNA of Toll\u2010Like\u2010Receptor\u20104 (TLR4), NLRP3, caspase 1, Interleukin\u20101<jats:italic>\u03b2<\/jats:italic> (IL1\u2010<jats:italic>\u03b2<\/jats:italic>), Interleukin\u201018 (IL\u201018), IL\u20106, IL\u20106\u2010receptor (IL\u20106R), and glycoprotein 130 (gp130) were isolated from thrombi and circulating leukocytes and relatively quantified by RT\u2010PCR. A part of each thrombus was embedded in paraffin for histology and immunohistochemistry analyses. <jats:italic>Results<\/jats:italic>. Genes encoding the 8 markers were present in 76\u2010100% of thrombi. Expression of TLR4 in thrombi significantly correlated to troponin T (<jats:italic>r<\/jats:italic> = 0.455, <jats:italic>p<\/jats:italic> = 0.013), as did NLRP3 (<jats:italic>r<\/jats:italic> = 0.468, <jats:italic>p<\/jats:italic> = 0.024). Troponin T correlated with expression in circulating leukocytes of TLR4 (<jats:italic>r<\/jats:italic> = 0.438, <jats:italic>p<\/jats:italic> = 0.011), NLRP3 (<jats:italic>r<\/jats:italic> = 0.420, <jats:italic>p<\/jats:italic> = 0.0149), and IL\u20101<jats:italic>\u03b2<\/jats:italic> (<jats:italic>r<\/jats:italic> = 0.394, <jats:italic>p<\/jats:italic> = 0.023). IL\u20106R expression in thrombi correlated significantly to troponin T (<jats:italic>r<\/jats:italic> = 0.434, <jats:italic>p<\/jats:italic> = 0.019), whereas gp130 was inversely correlated (<jats:italic>r<\/jats:italic> = \u22120.398, <jats:italic>p<\/jats:italic> = 0.050). IL\u20106 in circulating leukocytes correlated inversely to troponin T (<jats:italic>r<\/jats:italic> = \u22120.421, <jats:italic>p<\/jats:italic> = 0.015). There were no significant correlations between genes expressed in thrombi and time from symptom to PCI. <jats:italic>Conclusions<\/jats:italic>. The inflammasome signaling pathway was actively regulated in coronary thrombi and in circulating leukocytes from patients with STEMI, in association with myocardial damage measured by troponin T. This supports the strategy of medically targeting this pathway in treating myocardial infarction and contributes to sort out optimal timing and targets for anti\u2010inflammatory treatment. The study is registered at clinicaltrials.gov with identification number <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/clinicaltrials.gov\/ct2\/show\/NCT02746822\">NCT02746822<\/jats:ext-link>.<\/jats:p>","DOI":"10.1155\/2021\/5525917","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T14:24:16Z","timestamp":1622211856000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["The Inflammasome Signaling Pathway Is Actively Regulated and Related to Myocardial Damage in Coronary Thrombi from Patients with STEMI"],"prefix":"10.1155","volume":"2021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9680-9688","authenticated-orcid":false,"given":"Jostein","family":"Nordeng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2517-6382","authenticated-orcid":false,"given":"Hossein","family":"Schandiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3321-6572","authenticated-orcid":false,"given":"Svein","family":"Solheim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4068-2192","authenticated-orcid":false,"given":"Sissel","family":"\u00c5kra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1287-9174","authenticated-orcid":false,"given":"Pavel","family":"Hoffman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2698-2586","authenticated-orcid":false,"given":"Borghild","family":"Roald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1392-6481","authenticated-orcid":false,"given":"Bj\u00f8rn","family":"Bendz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0409-8792","authenticated-orcid":false,"given":"Harald","family":"Arnesen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5012-9214","authenticated-orcid":false,"given":"Ragnhild","family":"Helseth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4071-3358","authenticated-orcid":false,"given":"Ingebj\u00f8rg","family":"Seljeflot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,28]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehx393"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0b013e3182009701"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra071667"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14155"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2018.01.001"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1253\/circj.68.308"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/021849230301100316"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehu356"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2010.09.077"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2014.11.028"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01592"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2017.161"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.140"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1707914"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2011.01.034"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.4989"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/1396398"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehw171"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.306656"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.313129"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2014.01.007"},{"key":"e_1_2_9_23_2","first-page":"360","article-title":"Understanding interobserver agreement: the kappa statistic","volume":"37","author":"Viera A. 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