{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T18:53:54Z","timestamp":1780512834507,"version":"3.54.1"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T00:00:00Z","timestamp":1626307200000},"content-version":"vor","delay-in-days":195,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81501060"],"award-info":[{"award-number":["81501060"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81172848"],"award-info":[{"award-number":["81172848"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>Adiponectin (ADPN) plays an important role in cerebral ischemia\u2010reperfusion injury. Although previous studies have confirmed that ADPN pretreatment has a protective effect on ischemic stroke, the therapeutic effect of ADPN on ischemic stroke and the underlying mechanism are still unclear. In order to clarify these questions, focal transient cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in mice and ADPN was administered for three times at 6\u2009h, 24\u2009h, and 48\u2009h after reperfusion. Meanwhile, a virus\u2010delivered HIF\u20101<jats:italic>\u03b1<\/jats:italic> siRNA was used before ADPN administration. The infarct volume, neurological score, cellular apoptosis, and oxidative stress were assessed at 72\u2009h after reperfusion. The long\u2010term outcome of mice after stroke was recorded as well. The results indicated that ADPN treatment reduced the infarct volume (<jats:italic>P<\/jats:italic> = 0.032), neurological deficits (<jats:italic>P<\/jats:italic> = 0.047), cellular apoptosis (<jats:italic>P<\/jats:italic> = 0.041), and oxidative responses (<jats:italic>P<\/jats:italic> = 0.031) at 72\u2009h after MCAO. Moreover, ADPN increased both the protein level and transcriptional activity of HIF\u20101<jats:italic>\u03b1<\/jats:italic> as evidenced by the transcription levels of VEGF (<jats:italic>P<\/jats:italic> = 0.046) and EPO (<jats:italic>P<\/jats:italic> = 0.043) at 72\u2009h after MCAO. However, knockdown of HIF\u20101<jats:italic>\u03b1<\/jats:italic> partially reversed the antioxidant and treatment effect of ADPN after cerebral ischemia. In the observation of long\u2010term outcome after ADPN treatment, it demonstrated that ADPN not only prevented the cerebral atrophy (<jats:italic>P<\/jats:italic> = 0.031) and the neurological function decline (<jats:italic>P<\/jats:italic> = 0.048), but also promoted angiogenesis (<jats:italic>P<\/jats:italic> = 0.028) after stroke. In conclusion, our findings suggest that ADPN is effective in treatment of ischemic stroke which could be attributed to the increased antioxidant capacity regulated by HIF\u20101<jats:italic>\u03b1<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2021\/5531048","type":"journal-article","created":{"date-parts":[[2021,7,15]],"date-time":"2021-07-15T21:20:07Z","timestamp":1626384007000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Adiponectin Treatment Attenuates Cerebral Ischemia\u2010Reperfusion Injury through HIF\u20101<i>\u03b1<\/i>\u2010Mediated Antioxidation in 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