{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T15:57:09Z","timestamp":1787587029564,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"vor","delay-in-days":123,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20180182"],"award-info":[{"award-number":["BK20180182"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:p>\n                    <jats:italic>Introduction<\/jats:italic>\n                    . Reactive oxygen species (ROS) induced by extracellular cytokines trigger the expression of inflammatory mediators in osteoarthritis (OA) chondrocyte. Peroxisome proliferator\u2010activated receptor gamma (PPAR\n                    <jats:italic>\u03b3<\/jats:italic>\n                    ) exerts an anti\u2010inflammatory effect. The aim of this study was to elucidate the role of PPAR\n                    <jats:italic>\u03b3<\/jats:italic>\n                    in interleukin\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010 (IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010) induced cyclooxygenase\u20102 (COX\u20102) and prostaglandin E\n                    <jats:sub>2<\/jats:sub>\n                    (PGE\n                    <jats:sub>2<\/jats:sub>\n                    ) expression through ROS generation in OA chondrocytes.\n                    <jats:italic>Methods<\/jats:italic>\n                    . IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010induced ROS generation and chondrocyte apoptosis were determined by flow cytometry. Contents of NADPH oxidase (NOX), caspase\u20103, and caspase\u20109 were evaluated by biochemical detection. The involvement of NOX2 and mitogen\u2010activated protein kinases (MAPKs) in IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010induced COX\u20102 and PGE2 expression was investigated using pharmacologic inhibitors and further analyzed by western blotting. Activation of PPAR\n                    <jats:italic>\u03b3<\/jats:italic>\n                    was performed by using a pharmacologic agonist and was analyzed by western blotting.\n                    <jats:italic>Results<\/jats:italic>\n                    . IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010induced COX\u20102 and PGE\n                    <jats:sub>2<\/jats:sub>\n                    expression was mediated through NOX2 activation\/ROS production, which could be attenuated by N\u2010acetylcysteine (NAC; a scavenger of ROS), GW1929 (PPAR\n                    <jats:italic>\u03b3<\/jats:italic>\n                    agonist), DPI (diphenyleneiodonium chloride, NOX2 inhibitor), SB203580 (p38MAPK inhibitor), PD98059 (extracellular signal\u2010regulated kinase, ERK inhibitor), and SP600125 (c\u2010Jun N\u2010terminal kinase, JNK inhibitor). ROS activated p38MAPK to enter the nucleus, which was attenuated by PPAR\n                    <jats:italic>\u03b3<\/jats:italic>\n                    .\n                    <jats:italic>Conclusion<\/jats:italic>\n                    . In OA chondrocytes, IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    induced COX\u20102 and PGE\n                    <jats:sub>2<\/jats:sub>\n                    expression via activation of NOX2, which led to ROS production and MAPK activation. The activation of PPAR\n                    <jats:italic>\u03b3<\/jats:italic>\n                    exerted protective roles in the pathogenesis of OA.\n                  <\/jats:p>","DOI":"10.1155\/2021\/5551338","type":"journal-article","created":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T21:20:07Z","timestamp":1620163207000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["PPAR\n                    <i>\u03b3<\/i>\n                    Attenuates Interleukin\u20101\n                    <i>\u03b2<\/i>\n                    \u2010Induced Cell Apoptosis by Inhibiting NOX2\/ROS\/p38MAPK Activation in Osteoarthritis Chondrocytes"],"prefix":"10.1155","volume":"2021","author":[{"given":"Su","family":"Ni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai-Song","family":"Miao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1014-3369","authenticated-orcid":false,"given":"Chao","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,4]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.10.007"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60802-3"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms161125943"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rehab.2016.07.004"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvp400"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00804.x"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-12-0335"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2014-205743"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-014-1359-7"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2018.07.007"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2016.01.003"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1063-4584(03)00150-X"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M703318200"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2019.01.015"},{"key":"e_1_2_9_15_2","doi-asserted-by":"crossref","unstructured":"ZhuangC. 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